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Marine environmental DNA metabarcoding: understanding oceanic islands and mainland sites across anthropogenic gradients in two hemispheres

海洋环境 DNA 元条形码：了解两个半球跨人类活动梯度的海洋岛屿和大陆地点

基本信息

批准号：
20KK0164
负责人：
Reimer J・D
金额：
$ 11.65万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
财政年份：
2020
资助国家：
日本
起止时间：
2020-10-27 至 2024-03-31
项目状态：
已结题

项目摘要

In FY2020, our research was delayed by COVID-19. In short, the Australia side experienced several lockdowns, and Japan also had strict limits on entry and exit, making our planned field work in Australia along with inviting researchers to Japan impossible.Thus, we took a pragmatic course, and ordered the large majority of the equipment needed to implement the research, including a large BioBank deep freezer and small vacuum pumps we can use in the field. We also had numerous meetings with the Australian team, and planned for FY2021 (detailed below), including back-up plans in the case of continued COVID-19 problems.Finally, we continued to collaborate with our Australian counterparts on collected eDNA data from Okinawa, and published a scientific paper in Scientific Reports. This work was well received, and ranks #17 on most downloaded Ecology papers in the journal for 2020. The work examines marine metazoan diversity from eDNA at pristine and impacted sites in Okinawa, showing more fragmentation and less functional redundancy in more impacted sites. As well, the work notes that the majority of marine taxa experiencing turnover due to anthropogenic impacts are less well-studied groups such as sponges, annelids, and other so-called 'minor taxa'.

2020 财年，我们的研究因 COVID-19 而被推迟。总之，澳大利亚方面经历了多次封锁，日本方面也有严格的出入境限制，这使得我们计划在澳大利亚进行实地考察以及邀请研究人员来日本进行的工作变得不可能。因此，我们采取了务实的做法，订购了实施研究所需的大部分设备，包括大型BioBank深冷柜和我们可以在现场使用的小型真空泵。我们还与澳大利亚团队进行了多次会议，并为 2021 财年制定了计划（详细信息如下），包括在 COVID-19 问题持续存在的情况下的后备计划。最后，我们继续与澳大利亚同行合作，收集来自冲绳的 eDNA 数据，并在 Scientific Reports 上发表了一篇科学论文。这项工作广受好评，在该杂志 2020 年下载最多的生态学论文中排名第 17。这项工作检查了冲绳原始和受影响地点的 eDNA 海洋后生动物多样性，显示在受影响较大的地点有更多的碎片和更少的功能冗余。此外，该研究还指出，由于人为影响而经历更替的大多数海洋类群都是研究较少的类群，例如海绵、环节动物和其他所谓的“次要类群”。