Marine environmental DNA metabarcoding: understanding oceanic islands and mainland sites across anthropogenic gradients in two hemispheres

海洋环境 DNA 元条形码：了解两个半球跨人类活动梯度的海洋岛屿和大陆地点

• 批准号: 20KK0164
• 负责人: Reimer J・D
• 金额: $ 11.65万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-10-27 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20KK0164/
• 关键词: eDNA; marine ecosystem; marine biodiversity; anthropogenic impact; oceanic island; biodiversity; oceanic islands; anthropogenic pressure; marine ecosystems

In FY2020, our research was delayed by COVID-19. In short, the Australia side experienced several lockdowns, and Japan also had strict limits on entry and exit, making our planned field work in Australia along with inviting researchers to Japan impossible.Thus, we took a pragmatic course, and ordered the large majority of the equipment needed to implement the research, including a large BioBank deep freezer and small vacuum pumps we can use in the field. We also had numerous meetings with the Australian team, and planned for FY2021 (detailed below), including back-up plans in the case of continued COVID-19 problems.Finally, we continued to collaborate with our Australian counterparts on collected eDNA data from Okinawa, and published a scientific paper in Scientific Reports. This work was well received, and ranks #17 on most downloaded Ecology papers in the journal for 2020. The work examines marine metazoan diversity from eDNA at pristine and impacted sites in Okinawa, showing more fragmentation and less functional redundancy in more impacted sites. As well, the work notes that the majority of marine taxa experiencing turnover due to anthropogenic impacts are less well-studied groups such as sponges, annelids, and other so-called 'minor taxa'.

在2020财年，我们的研究被Covid-19延迟了。简而言之，澳大利亚方面经历了几次封锁，日本也严格限制进入和退出，这使我们在澳大利亚计划进行的现场工作以及邀请研究人员到日本的不可能。我们还与澳大利亚团队举行了许多会议，并计划在2021财年（下面详细介绍），其中包括持续Covid-19问题的备用计划。在本文中，我们继续与澳大利亚同行合作就Oukinawa收集的EDNA数据进行了合作，并在科学报告中发表了一份科学论文。这项工作受到了良好的接待，在2020年期刊上大多数下载的生态文件中排名第17。同样，这项工作指出，由于人为影响而导致的大多数海洋分类单元经历了流动率，这是较少的群体，例如海绵，annelids和其他所谓的“次要分类”。

项目成果

Australian Museum/University of Newcastle/Curtin University(オーストラリア)

澳大利亚博物馆/纽卡斯尔大学/科廷大学（澳大利亚）

Environmental DNA can act as a biodiversity barometer of anthropogenic pressures in coastal ecosystems

• DOI: 10.1038/s41598-020-64858-9
• 发表时间: 2020-05-20
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: DiBattista, Joseph D.;Reimer, James D.;Bunce, Michael
• 通讯作者: Bunce, Michael

University of Leeds(英国)

利兹大学（英国）

Victoria University of Wellington/Environmental Protection Authority(ニュージーランド)

惠灵顿维多利亚大学/环境保护局（新西兰）