What are the geometrical factors that regulate the propagation of calcium signals in astrocytes?

我在 2022 财年工作的模拟结果为微调大脑中神经元-星形胶质细胞通信的结构决定因素提供了新的见解。这项工作非常重要，因为由于纳米尺度研究生物过程的技术限制，此类研究无法在活体组织中进行。2022 财年开发的 CellScatter 软件将为计算科学家提供开放访问的真实 3D 细胞重建，以研究各种（病理）生理生物学条件。我们预计它将对细胞生物学和神经科学界产生重大影响，为未来关于健康和疾病生化过程的结构决定因素的实验和理论研究铺平道路。总体而言，这项工作揭示了复杂的细胞内纳米结构塑造健康和疾病中生化信号时空特性的机制。2022财年，这项工作在同行评审的国际期刊上发表了两篇文章（DOI： 10.1002/glia.24258 和 10.1007/s12031-022-02006-w)。另一份手稿正在接受同行评审（DOI：10.1101/2022.02.28.482292），另一份手稿正在准备中（Denizot 等人，2023 年，准备中）。我在国内和国际活动中展示了这些结果：2 次演讲（神经科学学会，在线，2022 年 11 月和日本神经科学学会第 45 届年会，日本，2022 年 6 月）和 1 幅海报（2022 年欧洲神经科学学会联合会论坛，法国巴黎，2022 年 7 月）。

The simulation results from my work in FY2022 provided novel insights into the structural determinants that finely tune neuron-astrocyte communication in the brain. This work is of high importance as such investigations cannot be performed in live tissue because of the technical limitations to study biological processes at the nanoscale.The CellScatter software developed during FY2022 will provide computational scientists with realistic 3D cell reconstructions in open-access to investigate various (patho-)physiological biological conditions. We expect that it will have a big impact on the cell biology and neuroscience communities, paving the way for future experimental and theoretical studies on the structural determinants of biochemical processes in health and disease.Overall, this work unraveled mechanisms by which the complex intracellular nano-architecture shapes the spatio-temporal properties of biochemical signals in health and disease.In FY2022, this work has led to the publication of 2 articles in peer-reviewed international journals (DOI: 10.1002/glia.24258 and 10.1007/s12031-022-02006-w). An additional manuscript is under peer review (DOI: 10.1101/2022.02.28.482292) and another is in preparation (Denizot et al., 2023, in prep.). I presented those results at both national and international events: 2 talks (Society for Neuroscience, online, Nov 2022 and the 45th Annual Meeting of the Japan Neuroscience Society, Japan, June 2022) and 1 poster (Federation of European Neuroscience Societies Forum 2022, Paris, France, July 2022).