What are the geometrical factors that regulate the propagation of calcium signals in astrocytes?

调节星形胶质细胞中钙信号传播的几何因素是什么？

• 批准号: 21F21733
• 负责人: DeSchutter Erik
• 金额: $ 0.96万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-07-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21F21733/
• 关键词: astrocyte; calcium signals; glia; modeling; reaction diffusion; 3D reconstruction; neuroscience; astrocytes; calcium signaling; interdisciplinary

The simulation results from my work in FY2022 provided novel insights into the structural determinants that finely tune neuron-astrocyte communication in the brain. This work is of high importance as such investigations cannot be performed in live tissue because of the technical limitations to study biological processes at the nanoscale.The CellScatter software developed during FY2022 will provide computational scientists with realistic 3D cell reconstructions in open-access to investigate various (patho-)physiological biological conditions. We expect that it will have a big impact on the cell biology and neuroscience communities, paving the way for future experimental and theoretical studies on the structural determinants of biochemical processes in health and disease.Overall, this work unraveled mechanisms by which the complex intracellular nano-architecture shapes the spatio-temporal properties of biochemical signals in health and disease.In FY2022, this work has led to the publication of 2 articles in peer-reviewed international journals (DOI: 10.1002/glia.24258 and 10.1007/s12031-022-02006-w). An additional manuscript is under peer review (DOI: 10.1101/2022.02.28.482292) and another is in preparation (Denizot et al., 2023, in prep.). I presented those results at both national and international events: 2 talks (Society for Neuroscience, online, Nov 2022 and the 45th Annual Meeting of the Japan Neuroscience Society, Japan, June 2022) and 1 poster (Federation of European Neuroscience Societies Forum 2022, Paris, France, July 2022).

我在2022财年的工作模拟结果为精细调节大脑中神经元-星形胶质细胞通讯的结构决定因素提供了新的见解。2022财政年度开发的CellScatter软件将为计算科学家提供开放获取的逼真3D细胞重建，以研究各种（病理）生理生物条件。我们预期该研究将对细胞生物学和神经科学领域产生重大影响，为未来有关健康和疾病中生化过程的结构决定因素的实验和理论研究铺平道路。整体而言，这项工作揭示了复杂的细胞内纳米结构塑造健康和疾病中生化信号时空特性的机制。于2022财政年度，这项工作已导致在同行评审的国际期刊上发表了2篇文章（DOI：10.1002/glia.24258和10.1007/s12031-022-02006-w）。另一篇手稿正在进行同行评审（DOI：10.1101/2022.02.28.482292），另一篇正在编写中（Denizot等人，2023年，编写中）。我在国家和国际活动中展示了这些结果：2场演讲（神经科学学会，在线，2022年11月和日本神经科学学会第45届年会，日本，2022年6月）和1张海报（欧洲神经科学学会联合会论坛2022年，巴黎，法国，2022年7月）。

项目成果

Disentangling astrocytic calcium signals: insights from spatially-extended models.

解开星形胶质细胞钙信号：来自空间扩展模型的见解。

• 发表时间: 2021
• 作者: T. P. Lyons;D. J. Gillard;C. Leblanc;J. Puebla;D. D. Solnyshkov;L. Klompmaker;I. A. Akimov;C. Louca;P. Muduli;A. Genco;M. Bayer;Y. Otani;G. Malpuech;& A. I. Tartakovskii;A. Denizot
• 通讯作者: A. Denizot

The endoplasmic reticulum in fine astrocytic processes: presence, shape, distribution and effect on Ca2+ activity

精细星形胶质细胞过程中的内质网：存在、形状、分布及其对 Ca2 活性的影响

• 发表时间: 2022
• 作者: A. Denizot;M. F. Veloz Castillo;P. Puchenkov;C. Cali;E. De Schutter.
• 通讯作者: E. De Schutter.

“Decoding the astrocytic calcium code with computational approaches.”

“用计算方法解码星形细胞钙密码。”

• 发表时间: 2022
• 作者: A. Denizot;E. De Schutter
• 通讯作者: E. De Schutter

ltrastructural and functional study of the endoplasmic reticulum in perisynaptic astrocytic processes

突触周围星形胶质细胞过程中内质网的超结构和功能研究

• 发表时间: 2022
• 作者: A. Denizot;M. F. Veloz Castillo;P. Puchenkov;C. Cali;E. De Schutter.
• 通讯作者: E. De Schutter.

The nanoscale morphology of astrocyte branchlets governs local calcium activity.

星形胶质细胞分支的纳米级形态控制着局部钙活性。

• 发表时间: 2021
• 作者: A. Denizot;M. Arizono;V. U. Nagerl;E. De Schutter;H. Berry.
• 通讯作者: H. Berry.