The Effect of Active Zone Protein Dynamics on Synaptic Vesicle Release Probability

活性区蛋白质动力学对突触小泡释放概率的影响

• 批准号: 20K06593
• 负责人: DeSchutter Erik
• 金额: $ 2.66万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K06593/
• 关键词: Synapse; Plasticity; Hippocampus; Computational biology; Systems biology

This year we have been focusing on finalising the software for its first public release. This has included adding some new features such as a new visualisation module in Blender, code modernisation and several optimisations. The vesicle development branch was merged into the main developmental master branch, meaning that this now become part of STEPS ready for public release of version 5.0. The software was extensively tested and validated, as has been summarised in a manuscript that has been uploaded to preprint server bioRxiv [1]. We are now finalising submission of this manuscript to a major journal.On the modelling side, all model simulations are complete, the manuscript [2] is nearing completion and it too will be soon submitted to a preprint server. In the manuscript we present a model of the complete synaptic vesicle cycle in unprecedented molecular and spatial detail and reveal how synaptic transmission is maintained during periods of high frequency and sustained stimulation. We are now preparing to submit these findings to a major journal.[1] I Hepburn, J Lallouette, W Chen, AR Gallimore, SY Nagasawa, E De Schutter. Hybrid vesicle and reaction-diffusion modeling with STEPS. bioRxiv 2023.05.08.539782; doi: https://doi.org/10.1101/2023.05.08.539782[2] AR Gallimore, I Hepburn, S. Rizzoli, E. De Schutter. Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle.

今年，我们一直专注于完成软件的首次公开发布。这包括添加一些新功能，例如Blender中的新可视化模块，代码现代化和一些优化。囊泡开发分支被合并到主开发主分支中，这意味着它现在成为STEPS的一部分，准备公开发布5.0版。该软件经过了广泛的测试和验证，如已上传至预印本服务器bioRxiv [1]的手稿中所总结。我们现在正在完成向一家主要期刊提交这份手稿的工作。在建模方面，所有的模型模拟都已完成，手稿[2]即将完成，它也将很快提交给预印本服务器。在手稿中，我们提出了一个完整的突触囊泡周期的模型，在前所未有的分子和空间细节，并揭示了突触传递是如何维持在高频率和持续刺激期间。我们现在正准备将这些发现提交给一家主要期刊。[1]I赫普本，J Lallouette，W Chen，AR Gallimore，SY Nagasawa，E De Schutter.混合囊泡和反应扩散建模与STEPS。bioRxiv 2023.05.08.539782; doi：https：//doi.org/10.1101/2023.05.08.539782 [2] AR Gallimore，I赫普本，S. Rizzoli，E.德舒特。完整突触囊泡周期详细空间模型中囊泡池的动态调节。

项目成果

Modeling the tripartite synapse.

建模三方突触。

• 发表时间: 2022
• 作者: Kuroda Junpei;Itabashi Takeshi;Iwane Atsuko H.;Aramaki Toshihiro;Kondo Shigeru;Toshihiro Aramaki;荒巻 敏寛;Toshihiro Aramaki;Erik De Schutter
• 通讯作者: Erik De Schutter