The Effect of Active Zone Protein Dynamics on Synaptic Vesicle Release Probability

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This year we have been focusing on finalising the software for its first public release. This has included adding some new features such as a new visualisation module in Blender, code modernisation and several optimisations. The vesicle development branch was merged into the main developmental master branch, meaning that this now become part of STEPS ready for public release of version 5.0. The software was extensively tested and validated, as has been summarised in a manuscript that has been uploaded to preprint server bioRxiv [1]. We are now finalising submission of this manuscript to a major journal.On the modelling side, all model simulations are complete, the manuscript [2] is nearing completion and it too will be soon submitted to a preprint server. In the manuscript we present a model of the complete synaptic vesicle cycle in unprecedented molecular and spatial detail and reveal how synaptic transmission is maintained during periods of high frequency and sustained stimulation. We are now preparing to submit these findings to a major journal.[1] I Hepburn, J Lallouette, W Chen, AR Gallimore, SY Nagasawa, E De Schutter. Hybrid vesicle and reaction-diffusion modeling with STEPS. bioRxiv 2023.05.08.539782; doi: https://doi.org/10.1101/2023.05.08.539782[2] AR Gallimore, I Hepburn, S. Rizzoli, E. De Schutter. Dynamic regulation of vesicle pools in a detailed spatial model of the complete synaptic vesicle cycle.