Novel WRC interacting receptors transducing signals at the membrane to the actin cytoskeleton

细胞形态的改变需要细胞膜重塑和肌动蛋白细胞骨架之间的动态相互作用，环境信号通过细胞骨架转化为形态和运动学上的变化。WAW蛋白是Arp2/3复合体的关键激活物，在许多细胞过程中协调肌动蛋白的成核作用，包括黏附、迁移和吞噬。波蛋白被结构性地增加到一个保守的、异五聚体的复合体，即控制其在时间和空间上的活动的波调节复合体(WRC)。我们最近描述了一种新的机制，通过在各种跨膜和膜相关蛋白中发现的保守的肽基序(WIRS)，WRC可以直接招募到膜上。这提供了一个新的信号平台，通过这个平台，环境信号可能转化为形态和运动性的变化。在这里，我们将分析果蝇卵子发生中的集体毛囊细胞迁移和创伤诱导的果蝇免疫细胞的定向单细胞迁移，并剖析这些线状表面受体是如何将配体与肌动蛋白细胞骨架的变化联系起来的。

Cell shape changes require a dynamic interplay between membrane remodeling and the actin cytoskeleton by which environmental signals are translated into changes in morphol-ogy and motility. The WASP family verprolin homologous (WAVE) protein is a key activator of the Arp2/3 complex and coordinates actin nucleation in many cellular processes including adhesion, migration and phagocytosis. The WAVE protein is constitutively incor-porated into a conserved, heteropentameric complex, the WAVE regulatory complex (WRC) that controls its activity in time and space. We recently described a novel mecha-nism by which the WRC can directly be recruited to membranes through a conserved pep-tide motif (WIRS) found in a variety of transmembrane and membrane-associated proteins. This provides a new signaling platform by which environmental signals might be translated into changes in morphology and motility. Here, we will analyze collective follicle cell migration in Drosophila oogenesis and wound-induced directed single cell migration of Drosophila immune cells and dissect how these WIRS surface receptors bridge ligands to changes of the actin cytoskeleton.