Chloroplast RNA polymerase activity during acclimation (A01)

适应过程中叶绿体 RNA 聚合酶活性 (A01)

• 批准号: 317539851
• 金额: --
• 依托单位: Abteilung Zellphysiologie
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/317539851?language=en
• 关键词: Chloroplast; RNA; polymerase; activity; during

This project aims to quantify transcriptional alterations that occur in chloroplasts during acclimation to changes in light or temperature. It will apply chloroplast nascent transcript sequencing strategies (GRO-seq and 5'-GRO-seq) in order to globally assess transcriptional contributions to changes in chloroplast gene expression during acclimation. By combining high-resolution quantitative transcriptional analyses with a reverse genetic approach we will determine distinct contributions of individual chloroplast RNA polymerases to transcriptional alterations.

该项目旨在量化叶绿体在适应光或温度变化过程中发生的转录变化。它将应用叶绿体新生转录本测序策略（GRO-seq和5'-GRO-seq）来全面评估在驯化过程中叶绿体基因表达变化的转录贡献。通过将高分辨率定量转录分析与反向遗传方法相结合，我们将确定单个叶绿体RNA聚合酶对转录改变的不同贡献。