Structural changes and inactivation of enzymes during drying and particle formation from levitated microdroplets

该项目的前两个目标保持不变，并增加了两个额外的目标1。完成升降器干燥和颗粒形成过程中酶分子聚集(层析)和失活(生化)变化的动力学测量。利用TDLAS对干燥速率进行光谱测量，以及二级结构元素变化的动力学，以及酶分子在干燥过程中的解折叠和升降器中的颗粒形成。描述流体流动、热传递/扩散、干燥和从蛋白质溶液的单个液滴形成颗粒的时间依赖问题的数值模型。还包括了展开的实验动力学数据(也包括二级结构元素的变化)以及聚集和失活的动力学数据。一个新的目标是蛋白质与其他配方成分、碳水化合物、氨基酸、聚合物和表面活性物质之间的相互作用。这些蛋白质在干燥过程中稳定下来，并在悬浮装置中形成颗粒。

The first two goals of the project remain unchanged and are extended by two additional goals.1. Complete the measurements of the kinetics of the changes in aggregation (chromatography) and inactivation (biochemical) of the enzyme molecules during drying and particle formation in the levitator.2. Spectroscopy measurements of drying rate using TDLAS, and the kinetics of the changes in secondary structure elements, and the unfolding of the enzyme molecules during drying and particle formation in the levitator.3. A numeric model to describe the time dependent problems of fluid flow, heat transport/diffusion, drying and particle formation from a single droplet of the protein solution. Also included are experimental kinetic data of unfolding (also changes in secondary structure elements) as well as data on the kinetics of aggregation and inactivity.4. A new goal is interactions between protein and other formulation components, carbon hydrate, amino acids, polymers and surface active agents. These stabilize of protein during drying and particle formation in the levitator.