Initiation of blood coagulation - Evaluating the relevance of specific surface functionalities using self assembled monolayers

血液凝固的启动 - 使用自组装单层评估特定表面功能的相关性

• 批准号: 31785435
• 负责人: Dr. Claudia Sperling
• 金额: --
• 依托单位: Leibniz-Institut für Polymerforschung Dresden (IPF)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/31785435?language=en
• 关键词: Initiation; blood; coagulation; Evaluating; relevance

This project intends to test the impact of recently elucidated pathways of coagulation in vivo on coagulation and blood cell activation at biomaterial surfaces. Targeting for a more rational design of blood compatible surfaces requires a deeper understanding of material ¿ blood interactions. Recent insights in coagulation processes, especially the involvement of cellular systems, have not yet been integrated in hemocompatibility research yet where the main focus is still set on the activation of the ¿contact system¿. Model surfaces of self assembled monolayers with different surface functionalities (-COOH, -OH, -CH3) as well as two-component-layers with varying fractions of these will be used in the study, as they allow the modification of single parameters. Previous results with this system indicated an enhanced platelet adhesion preferably on -CH3-surfaces, leukocytes on OH-terminated surfaces and a synergistic effect of -CH3- and COOH-groups on plasmatic coagulation activation. This indicates an influence of the cellular systems on the plasmatic coagulation what is not in the general concept of blood compatibility of surfaces. The system will be used to check systematically the effects of different surface chemistry on the coagulation activation, impact of tissue factor (extrinsic/ activator pathway), contact activation (intrinsic/ enhancer pathway) and cellular systems (blood platelets and leukocytes).

该项目旨在测试最近阐明的体内凝血途径对生物材料表面凝血和血细胞活化的影响。以更合理的血液相容性表面设计为目标，需要更深入地了解材料与血液的相互作用。最近对凝血过程的见解，特别是细胞系统的参与，尚未整合到血液相容性研究中，但其主要焦点仍然集中在“接触系统”的激活上。研究中将使用具有不同表面官能度（-COOH、-OH、-CH3）的自组装单层模型表面以及具有不同比例的双组分层，因为它们允许修改单个参数。该系统先前的结果表明，血小板粘附增强（优选在-CH3-表面上）、白细胞在OH末端表面上增强，以及-CH3-和COOH-基团对血浆凝血激活的协同作用。这表明细胞系统对血浆凝固的影响不属于表面血液相容性的一般概念。该系统将用于系统地检查不同表面化学对凝血激活、组织因子（外在/激活剂途径）、接触激活（内在/增强剂途径）和细胞系统（血小板和白细胞）的影响。