Core Unit - Genome signatures and integrated systems biology of pathogen-host interaction

核心单元 - 病原体-宿主相互作用的基因组特征和综合系统生物学

• 批准号: 319835486
• 负责人: Professor Dr. Alexander Goesmann
• 金额: --
• 依托单位: Institut für Bioinformatik und Systembiologie
• 依托单位国家: 德国
• 项目类别: Clinical Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/319835486?language=en
• 关键词: Core; Unit; Genome; signatures; integrated

Understanding cell-specific gene regulatory events and their impact on cellular networks in infected lungs requires an integrative CRU platform which delivers high-throughput transcriptomics, genomics and epigenetics data and analyses from primary lung cells/organoids/tissues including highly purified cell populations even of rare subsets, as well as advanced tools for data exploitation. The core unit Z1 provides service and support for processing murine and human lung specimens including patient samples to all CRU projects. This includes automated sample/library preparation, whole-transcriptome and miRNA analyses by microarrays and further next-generation sequencing support, including cutting-edge single cell applications for transcriptomics and epigenomics, as well as microbiome/metagenome analyses. In this regard, Z1 will continue to support the structured acquisition and storage of the generated experimental data for all CRU partners considering the FAIR principles. Furthermore, a comprehensive portfolio of bioinformatics software solutions and databases is offered to all CRU members to access, process, integrate and analyze data sets in a standardized and highly automated fashion. Finally, Z1 will continue to support all CRU members in planning experiments, statistical analysis, and interpretation and publication of results.

了解细胞特异性基因调控事件及其对感染肺部细胞网络的影响需要一个综合性CRU平台，该平台可提供高通量转录组学、基因组学和表观遗传学数据，并分析原代肺细胞/类器官/组织，包括高度纯化的细胞群，甚至罕见的亚群，以及先进的数据开发工具。核心单元Z1为所有CRU项目处理鼠和人肺标本（包括患者样本）提供服务和支持。这包括自动化样品/文库制备，通过微阵列进行全转录组和miRNA分析，以及进一步的下一代测序支持，包括转录组学和表观基因组学的尖端单细胞应用，以及微生物组/宏基因组分析。在这方面，Z1将继续支持所有CRU合作伙伴考虑FAIR原则，对生成的实验数据进行结构化采集和存储。此外，还为所有CRU成员提供全面的生物信息学软件解决方案和数据库组合，以便以标准化和高度自动化的方式访问、处理、集成和分析数据集。 最后，Z1将继续支持所有CRU成员规划实验、统计分析以及解释和发表结果。