Core Unit - Genome signatures and integrated systems biology of pathogen-host interaction

了解细胞特异性基因调控事件及其对感染肺部细胞网络的影响需要一个整合的CRU平台，该平台可以提供高通量转录组学、基因组学和表观遗传学数据，并分析来自原代肺细胞/类器官/组织，包括高度纯化的细胞群，甚至是罕见的亚群，以及用于数据开发的先进工具。核心单元Z1为所有CRU项目提供处理鼠和人肺标本（包括患者样本）的服务和支持。这包括自动化样品/文库制备，全转录组和miRNA分析，微阵列和进一步的下一代测序支持，包括转录组学和表观基因组学的尖端单细胞应用，以及微生物组/宏基因组学分析。在这方面，Z1将继续支持所有CRU合作伙伴根据FAIR原则对生成的实验数据进行结构化获取和存储。此外，为所有CRU成员提供全面的生物信息学软件解决方案和数据库组合，以标准化和高度自动化的方式访问，处理，集成和分析数据集。最后，Z1将继续支持所有CRU成员计划实验，统计分析，结果解释和发表。

Understanding cell-specific gene regulatory events and their impact on cellular networks in infected lungs requires an integrative CRU platform which delivers high-throughput transcriptomics, genomics and epigenetics data and analyses from primary lung cells/organoids/tissues including highly purified cell populations even of rare subsets, as well as advanced tools for data exploitation. The core unit Z1 provides service and support for processing murine and human lung specimens including patient samples to all CRU projects. This includes automated sample/library preparation, whole-transcriptome and miRNA analyses by microarrays and further next-generation sequencing support, including cutting-edge single cell applications for transcriptomics and epigenomics, as well as microbiome/metagenome analyses. In this regard, Z1 will continue to support the structured acquisition and storage of the generated experimental data for all CRU partners considering the FAIR principles. Furthermore, a comprehensive portfolio of bioinformatics software solutions and databases is offered to all CRU members to access, process, integrate and analyze data sets in a standardized and highly automated fashion. Finally, Z1 will continue to support all CRU members in planning experiments, statistical analysis, and interpretation and publication of results.