Core Unit - Genome signatures and integrated systems biology of pathogen-host interaction

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Understanding cell-specific gene regulatory events and their impact on cellular networks in infected lungs requires an integrative CRU platform which delivers high-throughput transcriptomics, genomics and epigenetics data and analyses from primary lung cells/organoids/tissues including highly purified cell populations even of rare subsets, as well as advanced tools for data exploitation. The core unit Z1 provides service and support for processing murine and human lung specimens including patient samples to all CRU projects. This includes automated sample/library preparation, whole-transcriptome and miRNA analyses by microarrays and further next-generation sequencing support, including cutting-edge single cell applications for transcriptomics and epigenomics, as well as microbiome/metagenome analyses. In this regard, Z1 will continue to support the structured acquisition and storage of the generated experimental data for all CRU partners considering the FAIR principles. Furthermore, a comprehensive portfolio of bioinformatics software solutions and databases is offered to all CRU members to access, process, integrate and analyze data sets in a standardized and highly automated fashion. Finally, Z1 will continue to support all CRU members in planning experiments, statistical analysis, and interpretation and publication of results.