Development and application of a nanofiltration process as membrane bioreactor for the production of galacto-oligosaccharides with increased degree of polymerization

纳滤工艺作为膜生物反应器的开发和应用，用于生产高聚合度的低聚半乳糖

• 批准号: 320646992
• 负责人: Dr.-Ing. Karina Altmann
• 金额: --
• 依托单位: Institut für Sicherheit und Qualität bei Milch und Fisch
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/320646992?language=en
• 关键词: Development; application; nanofiltration; process; membrane

Beta galactooligosaccharides (GOS) are beta glycosidically linked non digestible oligosaccharides composed of galactose with glucose or galactose at the reducing end and a degree of polymerization of 3 to 10 (mostly 3, 4 and 5). These oligosaccharides are synthesized from lactose by transgalactosylation activity of the enzyme beta galactosidase. Galactooligosaccharides exhibit biological activities. As prebiotics they promote the growth of commensal bacteria in the intestine, they modulate the immune system and inhibit the adhesion of pathogens on the gastrointestinal epithelial cell surface. A combination of the enzymatically production of galactooligosaccharides and a nanofiltration for the separation of the residual monosaccharides and disaccharides in a membrane bioreactor (MBR) could be a more efficient process for galactooligosaccharide production. Highly pure oligosaccharide preparations could be achieved within one process step. Moreover, the inhibition of the enzyme by high glucose and galactose concentrations could be decreased by the simultaneously separation of the monosaccharides from the glucose. Galactooligosaccharides with higher degree of polymerization could be synthesized. The main goal of the research work is the development and application of a nanofiltration process as membrane bioreactor for the production of galactooligosaccharides with increased degree of polymerization.The milestones of the research plan are: The establishment of a nanofiltration on a 2 to 3 L scale for the retention of oligosaccharides and removal of residual mono- and disaccharides by permeation through the membrane. The use of a nanofiltration system as membrane bioreactor to produce galacto-oligosaccharides with increased degree of polymerization. The use of the membrane bioreactor to produce galactosylated chitin oligosaccharides, and chitosan oligosaccharides with increased degree of polymerization. The instrumental analysis of oligosaccharides and the effect of oligosaccharides on pathogen adhesion. Advantages of the planned research work: The conversion of lactose to oligosaccharides with biological activities can lead to a valorization of the disaccharide. Chitin and chitosan, which should be applied as galactosyl acceptors, are inexpensive to produce and exhibit various biological activities (e.g. antibacterial, prebiotic, antiadhesive activities). The combination of the separate processes for the oligosaccharide production by enzymatic beta transgalactosylation and the nanofiltration for the purification of the product within one process in a membrane bioreactor could be more efficient.By permeation of the monosaccharides during oligosaccharide production in the membrane bioreactor the yield and the degree of polymerization of these sugars could be increased.

β-低聚半乳糖(GOS)是由半乳糖与葡萄糖或半乳糖在还原端，聚合度为3-10(主要是3、4、5)组成的糖苷键连接的非可消化低聚糖。这些低聚糖是由乳糖通过β-半乳糖苷酶的转半乳糖化作用合成的。低聚半乳糖具有生物活性。作为益生素，它们促进肠道内共生细菌的生长，调节免疫系统，抑制病原体在胃肠道上皮细胞表面的黏附。采用酶法生产低聚半乳糖和膜生物反应器(MBR)纳滤分离残留单糖和双糖相结合的方法可能是一种更有效的低聚半乳糖生产工艺。一步法可获得高纯度的低聚糖制剂。同时将单糖从葡萄糖中分离出来，可以减轻高浓度葡萄糖和半乳糖对酶的抑制作用。可以合成聚合度较高的低聚半乳糖。研究工作的主要目标是开发和应用纳滤法作为膜生物反应器来生产聚合度更高的低聚半乳糖。研究计划的里程碑是：建立一个2-3 L规模的纳滤器，用于保留低聚糖并通过膜渗透去除残留的单糖和双糖。使用纳滤系统作为膜生物反应器来生产聚合度更高的低聚半乳糖。利用膜生物反应器生产半乳糖化甲壳素低聚糖，以及聚合度提高的壳寡糖。低聚糖的仪器分析及低聚糖对病原菌黏附的影响。计划的研究工作的优点：将乳糖转化为具有生物活性的低聚糖可以导致双糖的价态。甲壳素和壳聚糖作为半乳糖受体具有生产成本低、具有多种生物活性(如抗菌、益生菌、抗粘连活性)等优点。在膜生物反应器中，将酶促β-转半乳糖化生产低聚糖的分离工艺和纳滤纯化产品的分离工艺相结合，可以更有效地提高低聚糖的产率和聚合度。