Anti-Müllerian-hormone and gonadotropin dependent changes in testis and pituitary of a cichlid fish, the Nile tilapia (Oreochromis niloticus).

丽鱼科鱼尼罗罗非鱼（Oreochromis niloticus）睾丸和垂体中抗苗勒氏管激素和促性腺激素依赖性变化。

• 批准号: 321375215
• 负责人: Dr. Frank Pfennig
• 金额: --
• 依托单位: Department of Animal Sciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/321375215?language=en
• 关键词: Anti; llerian; hormone; gonadotropin; dependent

Nile tilapia is an African cichlid and develops social hierarchies with dominant and subordinate animals. Dominant males have high levels of follicle stimulating hormone (FSH) and spermatogenesis is activated. A dominant individual can suppress the reproductive function of subordinates. Spermatogenesis of vertebrates is controlled by gonadotropins (like FSH) and androgens (like testosterone). The Anti-Müllerian-hormone (AMH) is an important regulator of germ cell development and steroid production in the gonads of vertebrates. AMH is differentially expressed in the testes from dominant and subordinate tilapia males. We want to explore the interrelations of these hormones. We use tilapia as a model fish with the aim to correlate changes in the social status with the endocrine and paracrine control of spermatogenesis. By the use of ex vivo testis-organ cultures we will investigate the influence of recombinant FSH, 11-Ketotestosteron (11-KT) and recombinant AMH on testis development. Expression and cytological analysis will be performed by quantitative PCR and immune-histological detection of selected key genes and by estimation of proliferation and apoptosis at the cellular level. Furthermore, the analysis of the transcriptome of the hormone-treated testis-cultures will be used to identify AMH target genes and to obtain information about FSH and 11-KT dependent or independent expression of AMH controlled genes. The identified differentially expressed genes of the treated organ cultures will be compared with the transcriptome data from dominant and subordinate males.Social dominance is accompanied by a hyperplasia of gonadotropic cells. AMH is also present outside of the gonads in brain and pituitary of Nile tilapia and is a strong candidate for regulation of cell proliferation and development. Therefore, we also intend to investigate the influence of AMH on gonadotropic cells by organotypic brain-slice cultures. AMH can be allocated also via the blood stream and by an AMH specific ELISA we will determine the serum level in dominant and subordinate males. The outcome of this project will give insight into hormonal control and the role of the social status and social stress on gonad development and reproduction in Nile tilapia, which is an economically important species. The results will also help to unravel the complex endocrine control of spermatogenesis in mammals.

尼罗罗非鱼是一种非洲慈鲷，并发展出社会等级制度，具有统治地位和从属地位。优势雄性具有高水平的卵泡刺激素（FSH），精子发生被激活。一个占支配地位的个体可以压制下属的生殖功能。脊椎动物的精子发生由促性腺激素（如FSH）和雄激素（如睾酮）控制。抗苗勒氏激素（Anti-Müllerian-hormone，AMH）是脊椎动物生殖细胞发育和性腺类固醇激素产生的重要调节因子。AMH在优势和从属罗非鱼雄性睾丸中的差异表达。我们想探索这些激素之间的相互关系。我们使用罗非鱼作为模式鱼，目的是将社会地位的变化与精子发生的内分泌和旁分泌控制相关联。本研究采用体外睾丸器官培养的方法，研究重组FSH、11-酮睾酮（11-KT）和重组AMH对睾丸发育的影响。将通过定量PCR和免疫组织学检测选定的关键基因，并通过估计细胞水平的增殖和凋亡，进行表达和细胞学分析。此外，将使用经FSH处理的睾丸培养物的转录组分析来鉴定AMH靶基因，并获得关于AMH控制基因的FSH和11-KT依赖性或独立性表达的信息。将处理的器官培养物中鉴定的差异表达基因与显性和从属雄性的转录组数据进行比较。社会优势伴随着促性腺细胞的增生。AMH也存在于尼罗罗非鱼的大脑和脑垂体的性腺之外，并且是调节细胞增殖和发育的强有力的候选者。因此，我们也打算研究AMH对促性腺激素细胞的影响，通过器官型脑片培养。AMH也可以通过血流分配，并通过AMH特异性ELISA，我们将确定占主导地位和从属地位的男性的血清水平。该项目的结果将深入了解荷尔蒙控制和社会地位和社会压力对尼罗罗非鱼性腺发育和繁殖的作用，尼罗罗非鱼是一种重要的经济物种。这些结果也将有助于阐明哺乳动物精子发生的复杂内分泌控制。