Analysis of key proteins involved in stomatal signaling using a novel nanobody technology.

气孔孔是由一对特殊的保卫细胞形成的，这些保卫细胞调节二氧化碳进入植物并使植物的蒸腾水分损失最小化。过去几十年的研究揭示了质子泵、钾离子通道和阴离子通道在调节气孔运动以响应环境胁迫方面的关键功能。然而，这些蛋白质在应激诱导表达过程中的蛋白质运输如何进行的关键问题仍然没有答案。这些问题将通过结合i)在植物中建立强大的创新抗体(纳米体)技术来解决，i)使用由共同主持G.Chang教授开发的新的纳米体技术，ii)对质子泵、K通道和阴离子通道SLAC1的免疫检测，以及iii)使用实时动力学来监控免疫标记的蛋白质对环境刺激的响应。我将继续从事博士后研究，并在加州大学圣地亚哥分校生物科学部的J.Schroeder教授和加州大学圣地亚哥分校医学院的G.Chang教授两个实验室接受出色的跨学科培训，以成功完成这个新颖而创新的项目。

Stomatal pores are formed by pairs of specialized guard cells regulating CO2 influx into plants and minimizing transpirational water loss of plants. Studies over the last decades revealed key functions of proton pumps, K+- and anion channels in regulating stomatal movement in response to environmental stresses. However, key questions into how protein trafficking of these proteins during stress-induced expression proceeds remain unanswered. These issues will be addressed by combining i) establishment of a powerful innovative antibody (nanobody) technology in plants using a novel nanobody technology developed by co-host Prof. Dr. G. Chang, ii) immunological detection of proton pumps, K+channels and the anion-channel SLAC1 and iii) monitoring protein trafficking of the immunologically labeled proteins using real time kinetics in response to environmental stimuli. I will pursue post doctoral research and receive excellent interdisciplinary training in the two laboratories of Prof. Dr. J. Schroeder (Division of Biological Sciences, UC San Diego) and Prof. Dr. G. Chang (School of Medicine, UC San Diego) to succeed in this novel and innovative project.