Development of Novel mRNA Delivery Systems based on Flexible Biodegradable Block Copolymers

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Here, we focused on the development of nanocarriers for safe and stable systemic delivery of mRNA. Thus, we have developed a library of polymers, and chemically modified with amino acids which could stable mRNA by hydrophobic interaction and π-π interaction. We confirmed that these polymers were biodegradable in physiological conditions. Polymeric micelles prepared by polymers with π-π interaction were found to be safe in vitro after incubation with 50% Fetal Bovine Serum, could achieve a highest mRNA expression in vitro. We found that micelles with π-π interaction with mRNA showed higher protein expression in the lung of mouse after 24 h intratracheal administration. Moreover, these micelles maintained over 10% of loaded mRNA in mice blood after 10 mins of intravenous administration.