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SWEETs: Mechanistic model of sucrose allocation in grains of barley and rice

SWEETs：大麦和稻米中蔗糖分配的机制模型

基本信息

批准号：
326548644
负责人：
Privatdozentin Dr. Ljudmilla Borisjuk
金额：
--
依托单位：
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/326548644?language=en
关键词：
SWEETs Mechanistic model sucrose allocation

项目摘要

Sucrose is the main source of carbon for growth and storage product synthesis in seeds. Our previous project identified HvSWEET11b and HvSWEET15a genes as key candidates for sucrose release in developing barley grains. We also verified their sucrose transport activity in vitro and localized their expression along the main sucrose allocation route. Now, we have generated transgenic barley plants with up and down regulated expression of SWEET genes, and plan their comprehensive characterization using our unique analytical (imaging) pipeline. We will apply magnetic resonance imaging (for surveying and monitoring of sucrose allocation in the living grain), infrared-based microspectroscopy (for high resolution quantitative sucrose mapping), RNASequencing, metabolite profiling and others for the analysis of developing grains. Moreover, concurrent molecular studies on rice revealed that OsSWEET11-1 and OsSWEET15-1 show all the hallmarks of being essential for grain ﬁlling in rice. Respective single and double mutant plants became available, and will also be analysed in the proposed project. This comparative analysis will solve the long-standing debate whether barley and rice exploit similar or distinct sucrose delivery routes. The project will exploit new genetic resources to study the genetic diversity in SWEET genes in genome wide association scans. Ultimately, a mechanistic view on sucrose import in grains of two important cereal crops will be provided as well as a knowledge base required for engineering of improved grain filling in cereals.

蔗糖是种子生长和贮藏产物合成的主要碳源。我们以前的项目确定了HvSWEET 11b和HvSWEET 15 a基因作为发育中的大麦籽粒中蔗糖释放的关键候选基因。我们还验证了它们的蔗糖转运活性在体外和本地化的表达沿着主要的蔗糖分配路线。现在，我们已经产生了转基因大麦植物与向上和向下调节表达的SWEET基因，并计划他们的全面表征使用我们独特的分析（成像）管道。我们将应用磁共振成像（用于调查和监测蔗糖在活谷物中的分配），基于红外线的显微光谱（用于高分辨率定量蔗糖绘图），RNAS测序，代谢产物分析等用于分析发育中的谷物。此外，对水稻的同步分子研究表明，OsSWEET 11 -1和OsSWEET 15 -1显示了水稻籽粒灌浆所必需的所有特征。已经有了相应的单突变体和双突变体植物，也将在拟议项目中进行分析。这种比较分析将解决长期存在的争论，大麦和水稻是否利用相似或不同的蔗糖输送途径。该项目将利用新的遗传资源，在全基因组关联扫描中研究SWEET基因的遗传多样性。最后，蔗糖进口的两个重要的谷类作物的谷物的机械观点将提供以及所需的知识基础工程的改善谷物灌浆。