The effects of luteotrophic and luteolytic factors on feline luteal cells (functional characterization)

黄体营养因子和黄体分解因子对猫黄体细胞的影响（功能表征）

• 批准号: 329674804
• 负责人: Privatdozentin Dr. Beate Braun
• 金额: --
• 依托单位: Leibniz-Institut für Zoo- und Wildtierforschung (IZW)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/329674804?language=en
• 关键词: effects; luteotrophic; luteolytic; factors; feline

The majority of felids are considered as endangered. In order to contribute to the conservation of species, the biological basis of reproduction must also be elucidated. The Leibniz Institute for Zoo and Wildlife Research (Leibniz-IZW) has therefore been studying the reproductive biology of various felids for many years. We were able to show that in lynxes, corpora lutea (CL) are still active after (pseudo-)pregnancy, which is a reproductive peculiarity. In the project, we are testing luteotropic and luteolytic factors on luteal cells of feline species. In the first part of this project on this topic, we recognized that the limitation and heterogeneity of the starting material led to a limited feasibility of experiments. Therefore, in the second part (continuation project) we would like to use the progenitor cells of the luteal cells for the development of long-term cell cultures and would like to use these characterized cells for luteinization in 3D models. We will then use the models to test the influence of reactive oxygen species (ROS) and antioxidant substances on luteal cell viability. Should we gain a better understanding of the control of CL viability through these studies, this will not only have relevance for felids, but could also be relevant for the treatment of CL insufficiencies in other species.

大多数猫科动物被认为是濒危物种。为了有助于物种的保护，还必须阐明繁殖的生物学基础。莱布尼茨动物园和野生动物研究所（Leibniz-IZW）因此多年来一直在研究各种猫科动物的生殖生物学。我们能够证明，在猞猁，黄体（CL）仍然活跃（假）怀孕后，这是一个生殖特性。在这个项目中，我们正在测试猫的黄体细胞的促黄体和溶黄体因子。在本项目的第一部分中，我们认识到起始材料的局限性和异质性导致实验的可行性有限。因此，在第二部分（继续项目）中，我们希望使用黄体细胞的祖细胞进行长期细胞培养，并希望使用这些特征化的细胞在3D模型中进行黄体化。然后，我们将使用该模型来测试活性氧（ROS）和抗氧化物质对黄体细胞活力的影响。如果我们通过这些研究更好地了解CL活力的控制，这不仅与猫科动物相关，而且也可能与其他物种的CL不稳定性治疗相关。