Post-translational regulation in the Arabidopsis bZIP factor-controlled gene expression and protein-protein interaction network

拟南芥 bZIP 因子控制的基因表达和蛋白质-蛋白质相互作用网络中的翻译后调控

• 批准号: 33401326
• 负责人: Professor Dr. Klaus Harter
• 金额: --
• 依托单位: Department Molekulare Systembiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/33401326?language=en
• 关键词: Post; translational; regulation; Arabidopsis; bZIP

In this part of the project we will work on the function of Arabidopsis group C ZIP factors (mainly bZIP9 and 63) as additional interaction partners for group S1 bZIPs in reprogramming the metabolism in response to environmental conditions (e.g. nutrients, hypoxia, salt stress). We focus particularly on the implications of bZIP63 and bZIP9 phosphorylation with respect to its function as a transcription factor within the C/S1 network. Furthermore, the project aims at identifying in planta target genes of bZIP63 using a ChIP-seq approach and isolating additional, non-bZIP interaction partners of bZIP63 and bZIP9 by using a BiFC-based two-hybrid screen in protoplasts and an ER-based yeast split-ubiquitin screen.

在该项目的这一部分中，我们将研究拟南芥C组ZIP因子（主要是bZIP 9和63）作为S1组bZIPs的额外相互作用伙伴在响应环境条件（例如营养，缺氧，盐胁迫）的代谢中的功能。我们特别关注bZIP 63和bZIP 9磷酸化对它作为C/S1网络中转录因子的功能的影响。此外，该项目旨在使用ChIP-seq方法鉴定bZIP 63的植物靶基因，并通过在原生质体中使用基于BiFC的双杂交筛选和基于ER的酵母分裂泛素筛选来分离bZIP 63和bZIP 9的额外的非bZIP相互作用伴侣。

项目成果

The interaction of the Arabidopsis response regulator ARR18 with bZIP63 mediates the regulation of PROLINE DEHYDROGENASE expression.

• DOI: 10.1093/mp/ssu074
• 发表时间: 2014-10
• 期刊: Molecular plant
• 影响因子: 27.5
• 作者: Manikandan Veerabagu;Tobias Kirchler;K. Elgass;Bettina Stadelhofer;M. Stahl;K. Harter;Virtudes Mira-Rodado-Virtudes-M