Functional analysis of the Arabidopsis two-component signalling network

拟南芥二元信号网络的功能分析

• 批准号: 50009001
• 负责人: Professor Dr. Klaus Harter
• 金额: --
• 依托单位: Abteilung für Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/50009001?language=en
• 关键词: Functional; analysis; Arabidopsis; two; component

Two-component systems (TCS) represent important signal perception, transduction and integration mechanisms in higher plants. A multitude of plant TCS elements have been identified within the last years in Arabidopsis including histidine kinases (HKs), histidine phosphotransfer proteins (HPs) and response regulators (RRs). Plant HKs perceive environmental or endogenous signals and transduce them through HPs to their cognate RRs which mediate the cellular processes via, for instance, the initiation of complex transcriptional cascades. Several TCS elements also function as molecular nodes in the cross-talk with non-TCS signal transduction systems. In the proposed project we will focus, on the one hand, on the analysis of the initial molecular events during the activation of Arabidopsis histidine kinases (AHKs) and of the functional dynamics of AHKs in respect to intramembrane and intracellular movement. On the other hand, we will employ the Arabidopsis transcription factor ARR2 for the identification of target genes which may initiate the complex transcriptional program induced by the TCS network. We are confident that our project will provide new insights into the initiation of TCS signalling and the realisation of developmental programs and integrated responses to the environment in Arabidopsis.

双组分系统(TCS)是高等植物重要的信号感知、转导和整合机制。近年来，在拟南芥中发现了大量的植物TCS元件，包括组氨酸激酶(HKS)、组氨酸磷酸转移蛋白(HPS)和反应调节因子(RR)。植物HKS感知环境或内源信号，并通过HPS将其转导到它们的同源RRs，RRs通过例如启动复杂的转录级联来调节细胞过程。一些TCS元件也在与非TCS信号转导系统的串扰中起到分子节点的作用。在拟议的项目中，我们一方面将重点分析拟南芥组氨酸激酶(AHKs)激活过程中的初始分子事件，以及AHKs在膜内和细胞内运动的功能动力学。另一方面，我们将利用拟南芥转录因子ARR2来鉴定可能启动TCS网络诱导的复杂转录程序的靶基因。我们相信，我们的项目将为启动TCS信号以及实现拟南芥的发展计划和对环境的综合反应提供新的见解。