Functional analysis of the Arabidopsis two-component signalling network

双组分系统（TCS）是高等植物重要的信号感知、转导和整合机制。近年来在拟南芥中发现了大量的TCS元件，包括组氨酸激酶（HKs）、组氨酸磷酸转移蛋白（HPs）和反应调节因子（RRs）。植物HK感知环境或内源性信号，并通过HP将其转换为其同源RR，后者通过例如启动复杂的转录级联来介导细胞过程。几个TCS元件也在与非TCS信号转导系统的串扰中起分子节点的作用。在拟议的项目中，我们将集中在一方面，在拟南芥组氨酸激酶（AHKs）的激活过程中的初始分子事件的分析和AHKs在膜内和细胞内运动的功能动力学。另一方面，我们将利用拟南芥转录因子ARR2来鉴定可能启动TCS网络诱导的复杂转录程序的靶基因。我们相信，我们的项目将提供新的见解TCS信号的启动和实现的发展计划和综合反应的环境中拟南芥。

Two-component systems (TCS) represent important signal perception, transduction and integration mechanisms in higher plants. A multitude of plant TCS elements have been identified within the last years in Arabidopsis including histidine kinases (HKs), histidine phosphotransfer proteins (HPs) and response regulators (RRs). Plant HKs perceive environmental or endogenous signals and transduce them through HPs to their cognate RRs which mediate the cellular processes via, for instance, the initiation of complex transcriptional cascades. Several TCS elements also function as molecular nodes in the cross-talk with non-TCS signal transduction systems. In the proposed project we will focus, on the one hand, on the analysis of the initial molecular events during the activation of Arabidopsis histidine kinases (AHKs) and of the functional dynamics of AHKs in respect to intramembrane and intracellular movement. On the other hand, we will employ the Arabidopsis transcription factor ARR2 for the identification of target genes which may initiate the complex transcriptional program induced by the TCS network. We are confident that our project will provide new insights into the initiation of TCS signalling and the realisation of developmental programs and integrated responses to the environment in Arabidopsis.