Isolation and molecular characterisation of primary cilia from neural stem cells

神经干细胞初级纤毛的分离和分子表征

• 批准号: 336717240
• 负责人: Dr. Francesca Ciccolini
• 金额: --
• 依托单位: Abteilung für Neurobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/336717240?language=en
• 关键词: Isolation; molecular; characterisation; primary; cilia

The ability to exit the cell cycle and undergo quiescence is key to protect the genetic information and maintain the function of adult stem cells. Therefore, the understanding of molecular and cel-lular mechanisms underlying the regulation of stem cell quiescence is a fundamental issue in the field. In this context, primary cilia, organelles on the surface of stem cells, are of special interest due to their essential role in signal transduction and in the regulation of cell cycle progression. The main aim of the current research project is to purify and fully characterize signalling components in primary cilia from neural stem cells (NSCs). To this end we will take advantage of mechanical, chemical and genetic approaches to detach and label primary cilia from neural precursors and purify primary cilia from the cell body of NSCs using flow cytometry. Defining the molecular composition of primary cilia in NSCs will provide new tools for the analysis of cilia function and diversity. It may also have clinical relevance, as it will provide new insights into the molecular mechanisms underlying the regulation of NSC quiescence essential to release their regenerative potential.

退出细胞周期和休眠的能力是保护遗传信息和维持成体干细胞功能的关键。因此，了解干细胞静止调控的分子和细胞机制是该领域的一个基本问题。在这种情况下，原纤毛，干细胞表面的细胞器，由于其在信号转导和细胞周期进程调节中的重要作用而受到特别关注。当前研究项目的主要目的是纯化和充分表征神经干细胞（NSCs）初级纤毛中的信号成分。为此，我们将利用机械、化学和遗传方法从神经前体中分离和标记原代纤毛，并利用流式细胞术从NSCs细胞体中纯化原代纤毛。确定NSCs中初级纤毛的分子组成将为分析纤毛的功能和多样性提供新的工具。它也可能具有临床意义，因为它将为释放其再生潜能所必需的NSC静止调节的分子机制提供新的见解。