Capturing highly dynamic beasts: Integrative characterisation of dynamic viral complexes using cryo-EM, NMR, MD, and molecular virology

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The goal of this project is to understand the structure and dynamics of the replicationmachinery in hepatitis C virus (HCV). This is an enormous structural challenge as itinvolves several challenging proteins and RNA. The distinct structural features ofvarious protein domains (membrane-associated/cytosolic, structured/disordered),coupled with their highly dynamic nature, all contribute to the enormous structuralchallenge of characterising this complex. The student will elucidate the structuraldetails of RNA replication in HCV by bringing together diverse approaches such asNuclear Magnetic Resonance spectroscopy (NMR), cryo-Electron Microscopy (cryo-EM),and Molecular Dynamics simulations (MD), with in-cell virology assays.