持続肺換気保存法を用いたドナー肺機能改善と長時間肺保存法の開発

持续肺通气保存法改善供肺功能及长期肺保存法的开发

• 批准号: 19K09040
• 负责人: 松田 安史
• 金额: $ 2.66万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19K09040/
• 关键词: 肺移植; 肺保存; 持続換気保存; 代謝; 組織代謝; 臓器保存; 肺換気

ブタ摘出肺を用いた実験群で、肺虚脱群（deflation群）、肺膨張群（inflation群）、持続換気群（ventilation群）に分ける。deflation群では摘出肺を虚脱したまま4℃で保存、inflation群では肺を膨張させたまま4℃で保存、ventilation群では人工呼吸器を用いて換気した状態で4℃で保存した。それぞれの肺を24時間保存した後、実験に供与した。24時間保存前後の組織中ATP濃度の測定を行った。各群の24時間保存後の肺組織１g中のATP濃度を求めたところ、24時間保存後の肺組織中ATP濃度はdeflation群およびinflation群で低下する傾向となり、ventilation群では上昇する傾向にあった。各群間における保存前後で有意な変化はなかった。保存前の３群間では有意差がなかったものの、保存後では、deflation群およびinflation群に比較して、ventilation群で有意に高値であった。これは、保存中に換気を行うことで保存肺での代謝が行われ組織中のATP濃度が維持されたことが示唆される。また、同様に保存前後の組織中乳酸濃度を検討した。肺保存前の組織中乳酸濃度に比較して、保存後の組織ではinflation群で組織中の乳酸濃度の上葉は認められないものの、deflation群およびventilation群では組織中乳酸濃度が有意に上昇した。また保存後の組織中乳酸濃度はinflation群に比較して、deflation群およびventilation群で有意に高かった。deflation群で乳酸濃度が高かったのは、嫌気性代謝が亢進して乳酸が酸性されたことが考えられた。また、ventilation群で乳酸濃度が高かったのは、保存中の換気運動により組織中の筋肉活動により結果乳酸が産生された可能性が考えられた。

The patients were divided into four groups: lung deficiency group (deflation group), lung deficiency group (inflation group), lung deficiency group (inflation group) and lung failure group (ventilation group). In the deflation group, the lungs were collapsed and preserved at 4 ℃, the lungs in the ventilation group were preserved at 4 ℃, and the artificial respirators were stored at 4 ℃ in the ventilation group. The lungs were stored for 24 hours, and then the lung was stored for 24 hours. Before and after saving for 24 hours, check the ATP metric in the organization to determine the number of lines. After 24 hours of preservation, 1 g of lung tissue was stored in 1 g of ATP, and after 24 hours of preservation, ATP in lung tissue was detected in deflation group, inflation group was in low concentration, and ventilation group was isolated in upward phase. There is an intention to change the storage schedule between groups before and after saving. Save the first 3 groups with the intention to save the inflation group, the deflation group, the ventilation group, the inflation group, and the ventilation group. In the process of saving and saving, you need to save the lungs on behalf of the lungs. In the organization, you can use the ATP to maintain the temperature. The lactate concentration in the tissue was measured before and after preservation. The lactate concentration in lung tissue was measured before preservation, the lactate concentration in inflation group was detected after preservation, and the lactate concentration in deflation group was significantly higher than that in deflation group. After preservation, the levels of lactate in inflation group and deflation group were significantly higher than those in ventilation group. In the deflation group, the lactic acid level was high, and the sex offspring was hyperactive. The results of lactic acid metabolism and the possibility of lactic acid production in the tissue of high lactic acid concentration, high

项目成果

Malignant solitary fibrous tumors of the left atrial endocardium

左心房心内膜恶性孤立性纤维瘤

• DOI: 10.1177/0218492321989206
• 发表时间: 2022
• 期刊: Asian Cardiovascular and Thoracic Annals
• 影响因子: 0.7
• 作者: Suzuki Yamato;Matsuda Yasushi;Saito Ryoko;Adachi Osamu;Okada Yoshinori
• 通讯作者: Okada Yoshinori

Pneumonia With Scedosporium apiospermum and Lomentospora prolificans in a Patient After Bilateral Lung Transplantation for Pulmonary Hypertension: A Case Report

肺动脉高压患者双肺移植后并发顶端孢子菌和多生球孢子菌肺炎：一例报告

• DOI: 10.1016/j.transproceed.2021.02.002
• 发表时间: 2021
• 期刊: Transplantation Proceedings
• 影响因子: 0.9
• 作者: Suzuki Yamato;Oishi Hisashi;Matsuda Yasushi;Noda Masafumi;Kumata Sakiko;Hayasaka Kazuki;Okada Yoshinori
• 通讯作者: Okada Yoshinori

Ex vivo lung CT findings may predict the outcome of the early phase after lung transplantation

离体肺CT结果可预测肺移植后早期的结果

• DOI: 10.1371/journal.pone.0233804
• 发表时间: 2020
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Oishi Hisashi;Noda Masafumi;Sado Tetsu;Matsuda Yasushi;Niikawa Hiromichi;Watanabe Tatsuaki;Sakurada Akira;Hoshikawa Yasushi;Okada Yoshinori
• 通讯作者: Okada Yoshinori

The new method for long-term hypothermic lung preservation with continuous ventilation

持续通气长期低温保存肺的新方法

• 发表时间: 2019
• 作者: 渡邉 健司;山本 滋;前田 訓子;西村 愛代;諌山 慧士朗;八木 美佳子;康 東天;岡 正朗;永野浩昭;水上 洋一;Yasushi Matsuda
• 通讯作者: Yasushi Matsuda

Living Donor Lobar Lung Transplant for a Patient With Lung Disease Caused by ABCA3 Gene Mutations: A Case Report

ABCA3 基因突变引起的肺病患者活体肺叶移植：病例报告

• DOI: 10.1016/j.transproceed.2022.07.020
• 发表时间: 2022
• 期刊: Transplantation Proceedings
• 影响因子: 0.9
• 作者: Kumata Sakiko;Matsuda Yasushi;Oishi Hisashi;Sado Tetsu;Niikawa Hiromichi;Watanabe Tatsuaki;Noda Masafumi;Hoshikawa Yasushi;Sakurada Akira;Saito-Koyama Ryoko;Niizuma Hidetaka;Kitazawa Hiroshi;Akiba Miki;Sasahara Yoji;Okada Yoshinori
• 通讯作者: Okada Yoshinori