Novel transcription factors in higher plant plastids

高等植物质体中的新型转录因子

• 批准号: 34691380
• 负责人: Professor Dr. Thomas Pfannschmidt
• 金额: --
• 依托单位: Institut für Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34691380?language=en
• 关键词: Novel; transcription; factors; higher; plant

Chloroplasts are the plant organelles in which photosynthesis takes place, the process which is the base for life on our planet. They possess own DNA which encodes important components of the photosynthetic apparatus and a chloroplast-own gene expression machinery. Regulation of this machinery depends on the import of nucleus- encoded regulatory factors; however, most of them are still unknown. Known basic components as RNA polymerases and sigma factors are not sufficient to explain the complex and dynamic transcription changes which occur during acclimation of plants to varying environmental conditions. Our results from biochemistry and bioinformatics indicate the existence of several eukaryotic-type transcription factors in plastids which appear to be responsible for such changes. The proposed project aims to identify and characterise such novel transcription factors in chloroplasts by combination of biochemical and molecular techniques. The results will be of high importance for our understanding of chloroplast gene expression and plant responses to the environment which has highly relevant for biotechnology applications in the field of sustainable biomass, energy and agriculture.

叶绿体是发生光合作用的植物细胞器，该过程是我们星球上生命的基础。它们拥有自己的DNA，编码光合机构的重要组成部分和叶绿体自己的基因表达机制。这一机制的调节依赖于核编码的调节因子的输入;然而，它们中的大多数仍然是未知的。已知的基本成分如RNA聚合酶和σ因子不足以解释植物适应不同环境条件过程中发生的复杂和动态的转录变化。生物化学和生物信息学的结果表明，质体中存在几种真核类型的转录因子，这些转录因子似乎是造成这种变化的原因。该项目旨在通过生物化学和分子生物学技术相结合的方法鉴定和鉴定叶绿体中的此类新型转录因子。这些结果对于我们理解叶绿体基因表达和植物对环境的反应具有重要意义，这与可持续生物质，能源和农业领域的生物技术应用高度相关。