Intersystem photosynthetic redox signals in retrograde chloroplast-to-nucleus communication of higher plant cells

高等植物细胞逆行叶绿体与细胞核通讯中的系统间光合氧化还原信号

• 批准号: 42287515
• 负责人: Professor Dr. Thomas Pfannschmidt
• 金额: --
• 依托单位: Institut für Botanik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/42287515?language=en
• 关键词: Intersystem; photosynthetic; redox; signals; retrograde

Environmental fluctuations in plant illumination affect the efficiency of the photosynthetic electron transport and induce characteristic changes in the redox state of plastoquinone and thioredoxin. These redox changes initiate molecular responses which acclimate the photosynthetic process to the residing conditions and help the plant to survive in an everchanging environment. It is known that this functional feed-back mechanism involves a complex signalling network which controls plastid and nuclear genes, but how this network functions is largely unknown. Especially the control of nuclear genes is complex since it requires (a) signalling cascade(s) which leave(s) the organelle. Redox signals, therefore, represent a novel class of retrograde signals. In microarrays we identified the target genes and the velocity of the signals indicating that redox signals integrate photosynthesis, respiration, carbohydrate metabolism and tetrapyrrole and lipid biosynthesis. We aim i) to demonstrate that this transcriptional regulation produce functional outcomes in the respective cellular processes and ii) to identify the involved signalling components by novel mutant screens and promoter-protein interaction assays.

植物光照的环境波动影响光合电子传递的效率，并诱导质体醌和硫氧还蛋白的氧化还原状态的特征变化。这些氧化还原变化引发分子反应，使光合作用过程适应生存条件，并帮助植物在不断变化的环境中生存。已知这种功能反馈机制涉及控制质体和核基因的复杂信号网络，但该网络如何发挥作用在很大程度上是未知的。特别是核基因的控制是复杂的，因为它需要离开细胞器的信号级联。因此，氧化还原信号代表一类新的逆行信号。在微阵列中，我们确定了靶基因和信号的速度，表明氧化还原信号整合光合作用，呼吸，碳水化合物代谢和四吡咯和脂质生物合成。我们的目标是：i）证明这种转录调控在各自的细胞过程中产生功能性结果; ii）通过新的突变体筛选和启动子-蛋白质相互作用测定来鉴定所涉及的信号传导组分。