Disentangling the role of migration, human transport and Wolbachia infection in the global invasion of a disease vector, the Asian Tiger mosquito (Aedes albopictus), using a genomic approach.

使用基因组方法阐明迁徙、人类运输和沃尔巴克氏体感染在疾病媒介亚洲虎蚊（白纹伊蚊）全球入侵中的作用。

• 批准号: 348864031
• 负责人: Dr. Ann-Christin Honnen
• 金额: --
• 依托单位: Department of Epidemiology and Public Health (EPH)
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/348864031?language=en
• 关键词: Disentangling; role; migration; human; transport

The Asian Tiger mosquito (Aedes albopictus) presently belongs to the most invasive species worldwide. It is now present on all continents except Antarctica, with most of its present distribution range having been invaded in the last 30-40 years. However, little is known about which genetic lineages contributed to the historical natural and more recent human-mediated invasions and how genetic variation is distributed and maintained in the newly established populations. Besides, previous research is confined to few areas only, and even less is known about the influence of Wolbachia-induced cytoplasmatic incompatibilities during invasions of the Asian Tiger mosquito, which might strongly influence its population genetic structure. These factors, jointly and individually, are of profound importance to project future invasions of this species and the associated risks of disease transmission. The project I propose is tailored to fill the existing knowledge gaps in the global phylogeography of Aedes albopictus, its invasion routes, its population structure and the possible influence of Wolbachia infections. More specifically, I will use ddRADseq, a state-of-the-art genomic tool, which will allow a genomewide resolution of the demographic history of this competent invader. This technique will also enable me to evaluate Wolbachia infection status and to determine the influence of this pathogen on the distribution of the mitochondrial lineages and their role in the distribution pattern of the Asian Tiger mosquito. The expected results will provide a strong foundation for the assessment of invasion risk and probable invasion routes and thus inform monitoring and management actions. Furthermore, a genomic database for future studies will be created.

亚洲虎蚊（白纹伊蚊）目前属于全球最具入侵性的物种。现在它出现在除南极洲以外的所有大陆上，其目前分布范围的大部分是在过去 30-40 年间被入侵的。然而，对于哪些遗传谱系促成了历史上的自然入侵和最近的人类介导的入侵以及遗传变异如何在新建立的种群中分布和维持，人们知之甚少。此外，之前的研究仅限于少数领域，对于亚洲虎蚊入侵过程中沃尔巴克氏体引起的细胞质不相容性的影响知之甚少，这可能会强烈影响其种群遗传结构。这些因素无论是共同还是单独，对于预测该物种未来的入侵以及相关的疾病传播风险都具有极其重要的意义。我提出的项目旨在填补白纹伊蚊全球系统发育地理学、其入侵途径、种群结构以及沃尔巴克氏体感染可能产生的影响方面的现有知识空白。更具体地说，我将使用 ddRADseq，这是一种最先进的基因组工具，它将允许对这个有能力的入侵者的人口历史进行全基因组解析。这项技术还将使我能够评估沃尔巴克氏体感染状态，并确定该病原体对线粒体谱系分布的影响及其在亚洲虎蚊分布模式中的作用。预期结果将为评估入侵风险和可能的入侵路线提供坚实的基础，从而为监测和管理行动提供信息。此外，还将创建用于未来研究的基因组数据库。