The branched Entner-Doudoroff pathway in Thermoproteus tenax: Enzymes, regulation and physiological function
Thermoproteus tenax 中的分支 Entner-Doudoroff 途径:酶、调节和生理功能
基本信息
- 批准号:35244511
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2007
- 资助国家:德国
- 起止时间:2006-12-31 至 2010-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The general understanding of central carbohydrate metabolism in Archaea comprises the presence of the non-nonphosphorylative Entner-Doudoroff (ED) pathway in (hyper)thermophilic Archaea and of the semi-phosphoryltive ED pathway in halophilic Archaea. However, a recent combined comparative genomics and biochemical approach revealed the presence of the semi-phosphorylative pathway in the hyperthermophilic Crenarchaeota Thermoproteus tenax and Sulfolobus solfataricus. Initial biochemical studies led to the identification of i) a novel type of gluconate dehydratase ii) a bifunctional 2-keto-3-deoxy-(6-phospho-)gluconate (KD(P)G) aldolase, iii) the first archaeal 2-keto-3-deoxygluconate (KDG) kinase and iv) a glycerate kinase. These results suggest the presence of both ED modifications (semi- and non-phosphorylative) and thus a branched ED pathway in these organisms. In T. tenax the suggested branched ED pathway is active in addition to the well studied Embden-Meyerhof-Parnas (EMP) modification and seems to be specific for glucose, whereas it represents the only “promiscuous” route for glucose and galactose degradation in S. solfataricus. The goal of this proposed study is to confirm the presence of the branched ED pathway in T. tenax, study its enzymes, their stereoselectivity/promiscuity, as well as the regulation and physiological function of the pathway. To gain an integrated understanding, the proposed studies will be combined in different collaborations with structural biology, phylogenetic studies as well as modern high-throughput approaches (proteomics/phosphorylomics, transcriptomics). This project will not only contribute to the understanding of the archaeal central carbohydrate metabolism and its regulation, but will aid to further unravel the metabolic complexity in all three domains of life.
对古细菌中心碳水化合物代谢的一般认识包括(超)嗜热古细菌中存在非非磷酸化的enterner - doudoroff (ED)途径,以及嗜盐古细菌中存在半磷酸化的ED途径。然而,最近一项结合比较基因组学和生化方法的研究发现,在嗜热的Crenarchaeota Thermoproteus tenax和Sulfolobus solfataricus中存在半磷酸化途径。最初的生化研究鉴定出i)一种新型的葡萄糖酸脱水酶ii)一种双功能的2-酮-3-脱氧-(6-磷酸-)葡萄糖酸(KD(P)G)醛缩酶iii)第一种古细菌2-酮-3-脱氧葡萄糖酸(KDG)激酶和iv)一种甘油激酶。这些结果表明,在这些生物体中存在ED修饰(半磷酸化和非磷酸化),因此存在分支ED途径。在T. tenax中,除了已被充分研究的Embden-Meyerhof-Parnas (EMP)修饰外,所建议的分支ED通路也很活跃,并且似乎对葡萄糖是特异性的,而它代表了S. solfataricus中葡萄糖和半乳糖降解的唯一“混杂”途径。本研究的目的是确认黄芪中分支ED通路的存在,研究其酶及其立体选择性/混杂性,以及该通路的调控和生理功能。为了获得全面的理解,拟议的研究将与结构生物学,系统发育研究以及现代高通量方法(蛋白质组学/磷酸化组学,转录组学)的不同合作相结合。该项目不仅有助于理解古细菌中枢碳水化合物代谢及其调控,而且将有助于进一步揭示生命中所有三个领域的代谢复杂性。
项目成果
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Professorin Dr. Bettina Siebers其他文献
Professorin Dr. Bettina Siebers的其他文献
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{{ truncateString('Professorin Dr. Bettina Siebers', 18)}}的其他基金
Functional and comparative genomics for the central carbohydrate metabolism of hyperthermophilic Archaea, presented in a new database
新数据库中提出了超嗜热古菌中央碳水化合物代谢的功能和比较基因组学
- 批准号:
5447818 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Priority Programmes
SCyCode - Unraveling the cyanobacterial carbon switch and its regulation using enzyme kinetic analyses and mathematical modeling
SCyCode - 使用酶动力学分析和数学模型揭示蓝藻碳开关及其调节
- 批准号:
415484216 - 财政年份:
- 资助金额:
-- - 项目类别:
Research Units
相似国自然基金
古菌Sulfolobus tokodaii甘油醛脱氢酶及其对非磷酸化Entner-Doudoroff糖酵解代谢的调控机制
- 批准号:30900039
- 批准年份:2009
- 资助金额:20.0 万元
- 项目类别:青年科学基金项目
相似海外基金
How do glycolytic routes and especially the newly discovered Entner-Doudoroff pathway contribute to the central carbon metabolism in cyanobacteria and do they play an essential role for carbon fixation and photosynthesis?
糖酵解途径,特别是新发现的 Entner-Doudoroff 途径如何促进蓝细菌的中心碳代谢?它们在碳固定和光合作用中发挥重要作用吗?
- 批准号:
367110946 - 财政年份:2017
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Is the Entner-Doudoroff pathway an overlooked glycolytic route in cyanobacteria and did cyanobacteria transfer its key enzyme via endosymbiotic gene transfer to plants?
Entner-Doudoroff 途径是蓝藻中被忽视的糖酵解途径吗?蓝藻是否通过内共生基因转移将其关键酶转移到植物中?
- 批准号:
275052541 - 财政年份:2015
- 资助金额:
-- - 项目类别:
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Physiology, Biochemistry and Molecular Genetics of Entner- Doudoroff Metabolism in Escherichia coli
大肠杆菌 Entner-Doudoroff 代谢的生理学、生物化学和分子遗传学
- 批准号:
0049043 - 财政年份:2000
- 资助金额:
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Physiology, Biochemistry and Molecular Genetics of Entner- Doudoroff Metabolism in Escherichia coli
大肠杆菌 Entner-Doudoroff 代谢的生理学、生物化学和分子遗传学
- 批准号:
9723593 - 财政年份:1997
- 资助金额:
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