权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Endophytes and invasive plants: How do endophytic fungi affect the growth, environmental tolerance and competitive success of invasive knotweed?

内生真菌和入侵植物：内生真菌如何影响入侵虎杖的生长、环境耐受性和竞争成功？

基本信息

批准号：
353306725
负责人：
Professor Dr. Oliver Bossdorf, Ph.D.
金额：
--
依托单位：
Lehrbereich für Evolutionäre Ökologie der Pflanzen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2017
资助国家：
德国
起止时间：
2016-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/353306725?language=en
关键词：
Endophytes invasive plants How do

项目摘要

Invasive plants are causing substantial ecological and economic problems, and it is therefore important to understand what makes these plants so competitive and dominant. There is now increasing evidence that soil microbes play a key role in many plant invasions. However, much of this evidence is rather coarse, and we often do not know which specific groups of microorganisms promote plant invasiveness. One particular group of soil microbes that is very diverse and widespread but still little understood are fungal endophytes of the order Sebacinales. These species are usually difficult to isolate and cultivate, and therefore opportunities for experimental tests of their ecological effects have so far been rare. Here, we propose to conduct a project that will test for the effects of fungal endophytes on the growth, environmental tolerance and invasion success of exotic knotweeds (Fallopia ssp.), some of the worst invasive plants worldwide. We will work with a Sebacinales strain, Sebacina herbamans, that was recently isolated in our group. We know from preliminary work that S. herbamans can strongly affect plant growth, that it occurs in invasive knotweed, and that we are able to experimentally inoculate knotweed with S. herbamans. We also know that some (unknown) soil microbes promote dominance of invasive knotweed over native plants. Our project will consist of three complementary work packages: (1) A lab experiment in which we will test the effects of the endophytes on the growth of invasive knotweed under highly controlled conditions. (2) A more ecologically realistic garden experiment where we will test the effects of endophytes on the competitive ability and invasion success of knotweed in native plant communities under a range of different environmental conditions. (3) A field survey in which we will attempt to associate endophyte diversities and abundances with knotweed invasion success in the field. In the first two work packages, we will experimentally inoculate knotweed plants with S. herbamans, and we generally verify the success of these inoculations, and quantify endophyte colonization rates, through microscopy and quantitative real-time PCR. In the last work package, we will use next-generation sequencing methods to compare not only the distribution of S. herbamans but more broadly the diversity of all root-associated fungi across a broad range of invasive knotweed populations. Our project will uniquely combine invasion biology with endophyte research, and we hope that it will provide novel insights both in the ecology of endophytes and the determinants of plant invasion success.

入侵植物正在造成严重的生态和经济问题，因此了解是什么使这些植物如此具有竞争力和主导地位是很重要的。越来越多的证据表明，土壤微生物在许多植物入侵中起着关键作用。然而，这些证据中的大部分是相当粗糙的，我们通常不知道哪些特定的微生物群体促进了植物的入侵。土壤微生物的一个特殊群体是非常多样和广泛的，但仍然很少了解的是Sebacinales目的真菌内生菌。这些物种通常很难分离和培养，因此，迄今为止，对其生态影响进行实验测试的机会很少。在这里，我们建议进行一个项目，将测试真菌内生菌对生长，环境耐受性和外来虎杖（Fallopia ssp.）入侵成功的影响，一些世界上最严重的入侵植物。我们将使用我们组最近分离的Sebacinales菌株Sebacina herbamans。我们从初步工作中得知S. herbamans可以强烈影响植物生长，它发生在入侵的虎杖，我们能够实验虎杖与S。草药。我们还知道，一些（未知的）土壤微生物促进了入侵的虎杖对本地植物的优势。我们的项目将包括三个互补的工作包：（1）一个实验室实验，我们将在高度控制的条件下测试内生菌对入侵虎杖生长的影响。(2)一个更生态现实的花园实验，我们将测试内生菌的竞争能力和入侵成功的原生植物群落中的虎杖在一系列不同的环境条件下的影响。(3)一项实地调查，我们将试图将内生菌的营养和丰度与虎杖入侵成功的领域。在前两个工作包中，我们将实验性地用S. herbamans，我们通常验证这些接种的成功，并通过显微镜和定量实时PCR量化内生菌定殖率。在最后一个工作包中，我们将使用下一代测序方法不仅比较S。herbamans，但更广泛地说，所有根相关真菌的多样性，在广泛的入侵虎杖种群。我们的项目将独特的联合收割机入侵生物学与内生菌研究相结合，我们希望它将提供新的见解，无论是在内生菌的生态学和植物入侵成功的决定因素。