Characterization of a 5-formylcytosine chromocenter in Xenopus embryos
非洲爪蟾胚胎 5-甲酰胞嘧啶染色中心的表征
基本信息
- 批准号:354741800
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2017
- 资助国家:德国
- 起止时间:2016-12-31 至 2020-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DNA methylation is an epigenetic mark, which plays important roles in development and disease. It is now widely accepted that DNA methylation is dynamic and is subject to enzymatic demethylation. The Tet family of methylcytosine dioxygenases convert 5-methylcytosine to oxidized derivatives, which are intermediates towards active DNA demethylation, but which may also function as epigenetic marks in their own right. However, little is known about the function of oxidized cytosine derivatives as epigenetic marks and as to their role in vertebrate development. We have discovered that early Xenopus embryos display single nuclear foci of 5-formylcytosine (5fC) during the midblastula transition. We have evidence that these 5fC chromocenters are made up of repetitive DNA sequences residing on different chromosomes, which coalesce during interface. We now propose to characterize the nature, the function, and a possible biological role of this 5fC chromocenter in Xenopus development.
DNA甲基化是一种表观遗传标记,在发育和疾病中起重要作用。现在普遍认为DNA甲基化是动态的,并且受到酶促去甲基化的影响。甲基胞嘧啶双加氧酶的泰特家族将5-甲基胞嘧啶转化为氧化衍生物,其是活性DNA去甲基化的中间体,但其本身也可以作为表观遗传标记起作用。然而,人们对氧化胞嘧啶衍生物作为表观遗传标记的功能及其在脊椎动物发育中的作用知之甚少。我们已经发现,早期非洲爪蟾胚胎显示5-甲酰胞嘧啶(5 fC)的单核灶在中囊胚过渡。我们有证据表明,这些5 fC色心是由位于不同染色体上的重复DNA序列组成的,这些序列在界面期间合并。我们现在提出的性质,功能,和一个可能的生物学作用,这5 fC色心在非洲爪蟾的发展。
项目成果
期刊论文数量(8)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
GADD45 promotes locus-specific DNA demethylation and 2C cycling in embryonic stem cells
- DOI:10.1101/gad.325696.119
- 发表时间:2019-06
- 期刊:
- 影响因子:10.5
- 作者:Katrin M. Schüle;Manuel Leichsenring;Tommaso Andreani;Viviana Vastolo;Medhavi Mallick;M. Musheev;Emil Karaulanov;C. Niehrs
- 通讯作者:Katrin M. Schüle;Manuel Leichsenring;Tommaso Andreani;Viviana Vastolo;Medhavi Mallick;M. Musheev;Emil Karaulanov;C. Niehrs
The tumor suppressor PTPRK promotes ZNRF3 internalization and is required for Wnt inhibition in the Spemann organizer
- DOI:10.7554/elife.51248
- 发表时间:2020-01-14
- 期刊:
- 影响因子:7.7
- 作者:Chang, Ling-Shih;Kim, Minseong;Niehrs, Christof
- 通讯作者:Niehrs, Christof
GADD45A binds R-loops and recruits TET1 to CpG island promoters
- DOI:10.1038/s41588-018-0306-6
- 发表时间:2019-02-01
- 期刊:
- 影响因子:30.8
- 作者:Arab, Khelifa;Karaulanov, Emil;Niehrs, Christof
- 通讯作者:Niehrs, Christof
NEIL1 and NEIL2 DNA glycosylases protect neural crest development against mitochondrial oxidative stress
- DOI:10.7554/elife.49044
- 发表时间:2019-09-30
- 期刊:
- 影响因子:7.7
- 作者:Han, Dandan;Schomacher, Lars;Niehrs, Christof
- 通讯作者:Niehrs, Christof
The origin of genomic N6-methyl-deoxyadenosine in mammalian cells
- DOI:10.1038/s41589-020-0504-2
- 发表时间:2020-03-23
- 期刊:
- 影响因子:14.8
- 作者:Musheev, Michael U.;Baumgaertner, Anne;Niehrs, Christof
- 通讯作者:Niehrs, Christof
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Professor Dr. Christof Niehrs, Ph.D.其他文献
Professor Dr. Christof Niehrs, Ph.D.的其他文献
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{{ truncateString('Professor Dr. Christof Niehrs, Ph.D.', 18)}}的其他基金
Role of NEIL DNA glycosylases in DNA demethylation and differentiation
NEIL DNA 糖基化酶在 DNA 去甲基化和分化中的作用
- 批准号:
319444698 - 财政年份:2016
- 资助金额:
-- - 项目类别:
Research Grants
Roles and mechanisms of asymmetric LRP6 localization in Xenopus embryonic cells
非洲爪蟾胚胎细胞中 LRP6 不对称定位的作用和机制
- 批准号:
214902591 - 财政年份:2012
- 资助金额:
-- - 项目类别:
Research Units
Role of Gadd45 in pluripotency and murine embryogenesis
Gadd45 在多能性和小鼠胚胎发生中的作用
- 批准号:
64735175 - 财政年份:2008
- 资助金额:
-- - 项目类别:
Priority Programmes
System level analysis of the BMP4 synexpression groups
BMP4 同表达组的系统水平分析
- 批准号:
5403306 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Research Grants
Analyse der Genregulation in Synexpressionsgruppen
同表达组的基因调控分析
- 批准号:
5185846 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Research Grants
Die biologische Rolle und der Wirkungsmechanismus von R-sponding2
R-sponding2的生物学作用及作用机制
- 批准号:
5343057 - 财政年份:2001
- 资助金额:
-- - 项目类别:
Research Grants
Analyse der Genregulation in Synexpressionsgruppen
同表达组的基因调控分析
- 批准号:
5185848 - 财政年份:1999
- 资助金额:
-- - 项目类别:
Priority Programmes
Systematische Analyse embryonaler Genexpression in Xenopus
非洲爪蟾胚胎基因表达的系统分析
- 批准号:
5377499 - 财政年份:1997
- 资助金额:
-- - 项目类别:
Priority Programmes
相似海外基金
Programmable and Chemoselective Protein-DNA Crosslinking for Sensitive Detection of 5-Formylcytosine
用于灵敏检测 5-甲酰胞嘧啶的可编程化学选择性蛋白质-DNA 交联
- 批准号:
223355544 - 财政年份:2012
- 资助金额:
-- - 项目类别:
Priority Programmes