From stem cell activity to shoot morphology

从干细胞活性到芽形态

• 批准号: 357422635
• 负责人: Professor Dr. Jan Lohmann
• 金额: --
• 依托单位: Forschungsgruppe Stammzellbiologie
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/357422635?language=en
• 关键词: stem; cell; activity; shoot; morphology

Plant form is dependent on the activity of continuously active stem cell systems, called meristems. While it is well established that dynamic signaling cascades are central for setting up stable patterns that can be translated into functional morphologies, much less is known about the contribution of basic cellular parameters, such as cell size, cell shape or cell mechanics. Building on our results from the first FOR2581 funding phase, we will now address these issues by leveraging the Big Cells in Epidermis (bce) mutant we have isolated and characterized. The causal gene for the bce phenotype is a ribosomal RNA processing factor and we will hence study how defects in ribogenesis give rise to large and unevenly shaped cells under aim 1. In aim 2, we will use the bce mutant as a tool to study the role of cell size, volume and mechanics in morphogenetic processes from the molecular to the tissue level. Finally, we will ask how cellular parameters in defined domains of the meristem contribute to shoot morphogenesis and how physical cell state impinges on cell decision making by autonomous and non-autonomous mechanisms in aim 3. We will work closely with other groups of FOR2581 in areas including image segmentation, recording of mechanical features of cells and mathematical modelling to synthesize our multilevel data into testable hypotheses and predictions. Taken together, this proposal will leverage the bce mutant characterized in the first funding phase to advance our understanding of the interplay of basic cellular parameters, such as cell size, shape or mechanics, with well-studied signaling systems that act in concert to give rise to stable morphogenetic patterns of the shoot.

植物的形态取决于不断活跃的干细胞系统的活动，称为分生组织。 虽然已经确定动态信号级联是建立稳定模式的核心，这些模式可以转化为功能形态，但对基本细胞参数（如细胞大小、细胞形状或细胞力学）的贡献知之甚少。基于我们第一个FOR 2581资助阶段的结果，我们现在将通过利用我们分离和表征的表皮大细胞（bce）突变体来解决这些问题。bce表型的致病基因是核糖体RNA加工因子，因此我们将研究核糖合成缺陷如何导致目标1下的大而形状不均匀的细胞。在目标2中，我们将使用bce突变体作为工具，从分子到组织水平研究细胞大小、体积和力学在形态发生过程中的作用。最后，我们将问细胞参数在定义域的分生组织有助于拍摄形态和物理细胞状态如何影响细胞决策的自主和非自主机制的目标3。我们将与FOR2581的其他小组在图像分割、记录细胞机械特征和数学建模等领域密切合作，将我们的多级数据合成为可检验的假设和预测。总而言之，这个提议将利用第一个资助阶段的bce突变体来推进我们对基本细胞参数（如细胞大小、形状或力学）与经过充分研究的信号系统相互作用的理解，这些信号系统协同作用，产生稳定的芽形态发生模式。