Role of the bZIP transcription factor PERIANTHIA in the regulatory network underlying meristem function and floral patterning

bZIP转录因子PERIANTHIA在分生组织功能和花图案的调控网络中的作用

• 批准号: 49906311
• 负责人: Professor Dr. Jan Lohmann
• 金额: --
• 依托单位: Forschungsgruppe Stammzellbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49906311?language=en
• 关键词: Role; bZIP; transcription; factor; PERIANTHIA

This proposal is aimed at elucidating the role of PERIANTHIA (PAN), a bZIP transcription factor, in the regulatory network that underlies meristem function and floral patterning on a mechanistic level. PAN is active both in the shoot apical meristem as well as in young floral primordia, where it is necessary for the correct specification of perianth organ number. We have found that in addition to this role, PAN has important functions in floral development by activating AGAMOUS (AG), a core regulator necessary for patterning as well as stem cell control. Furthermore, pan mutations affect the expression of central players of the stem cell system in the shoot apical meristem. We are now proposing to study how PAN is able to control these two very diverse functions, namely cell proliferation in the shoot meristem and cell differentiation in emerging flowers. The specific aims are: (1) Identification of PAN targets by expression profiling and genome-wide binding studies. (2) Identification and analysis of PAN co-factors. (3) Discovery of novel genes in the PAN network. (4) Integration of PAN into known regulatory networks of meristem control and floral patterning. (5) Mechanistic understanding of PAN regulation. The proposed project will substantially advance our understanding of the regulatory network underlying meristem function and floral patterning and elucidate how can PAN act as a molecular switch between proliferation and differentiation of meristematic cells. On the infrastructure level, the community will not only benefit from the large-scale datasets produced, but more importantly from the advancements in chromatin-immunoprecipitation with microarray detection technologies we will achieve during the project. These technologies will be made available to other AFGN/NSF2010 groups immediately, even prior to publication.

该提案旨在阐明 PERIANTHIA (PAN)（一种 bZIP 转录因子）在调控网络中的作用，该调控网络是分生组织功能和花图案的机制水平。 PAN 在茎尖分生组织和幼小的花原基中都很活跃，这对于正确确定花被器官的数量是必要的。我们发现，除了这一作用之外，PAN 通过激活 AGAMOUS (AG) 在花发育中发挥重要作用，AGAMOUS (AG) 是图案形成和干细胞控制所必需的核心调节因子。此外，泛突变影响茎尖分生组织中干细胞系统核心参与者的表达。我们现在提议研究 PAN 如何能够控制这两种截然不同的功能，即芽分生组织中的细胞增殖和新兴花朵中的细胞分化。具体目标是：（1）通过表达谱和全基因组结合研究鉴定PAN靶标。 (2)PAN辅助因子的鉴定与分析。 (3)PAN网络中新基因的发现。 (4)将PAN整合到已知的分生组织控制和花图案的调控网络中。 (5)PAN调控的机理认识。拟议的项目将大大增进我们对分生组织功能和花图案背后的调控网络的理解，并阐明 PAN 如何充当分生组织细胞增殖和分化之间的分子开关。在基础设施层面，社区不仅将受益于所产生的大规模数据集，更重要的是受益于我们将在项目期间实现的微阵列检测技术染色质免疫沉淀方面的进步。这些技术将立即提供给其他 AFGN/NSF2010 小组，甚至在发布之前。