Structural and functional characterization of complex protein modules involved in regulation of proteasomal activitiy (B04)

参与蛋白酶体活性调节的复杂蛋白质模块的结构和功能表征（B04）

• 批准号: 35777298
• 金额: --
• 依托单位: Lehrstuhl für Biochemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/35777298?language=en
• 关键词: Structural; functional; characterization; complex; protein

Understanding of modulation and regulation of proteasomal activity cannot be complete without structural information about proteins involved. So far structure, function and mechanism have been elucidated only for 20S proteasome and few regulatory components. Our aim is to determine the structure of the lid and the signalosome complex, playing major role in the first steps of recognition and degradation of folded proteins. We want to combine and expand structural methods for characterization of major compounds of the protein degradation machinery and to provide mechanistic and modular insights into its regulation.

如果没有有关蛋白质的结构信息，对蛋白酶体活性调节和调节的理解就不可能完整。迄今为止，仅阐明了20S蛋白酶体的结构、功能和机制，而调控元件很少。我们的目标是确定盖子和信号体复合物的结构，它们在折叠蛋白识别和降解的第一步中发挥着重要作用。我们希望结合和扩展用于表征蛋白质降解机制的主要化合物的结构方法，并为其调节提供机械和模块化的见解。