The role of arbuscular mycorrhizas (AM) in the establishment of rainforest tree seedlings, and the application of AM fungi for reforestation approaches

丛枝菌根 (AM) 在雨林树苗建立中的作用以及 AM 真菌在重新造林方法中的应用

• 批准号: 36004602
• 负责人: Professor Dr. Arthur Schüßler
• 金额: --
• 依托单位: Universidad Técnica Particular de Loja (UTPL)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/36004602?language=en
• 关键词: role; arbuscular; mycorrhizas; AM; establishment

The 215 described species of Glomeromycota represent only a small fraction of their species diversity. Up to now, molecular ecological community analyses on arbuscular mycorrhizal fungi (AMF, Glomeromycota) mostly were lacking species level resolution, and they were often strongly biased by the methods used. We are introducing improved methods to solve the pitfalls of molecular AMF monitoring. To understand functional roles of the obligate symbiotic AMF in native Ecuadorian trees and to make AMF applicable in reforestation, we will: 1) perform molecular monitoring of AMF in natural forest seedlings (baseline data); 2) define the AMF established in previously planted trees in >4 years old plots (baseline data); 3) monitor the AMF succession in direct seeded tree seedlings (reforestation); 4) evaluate the potential of AMF inoculation for improving the performance of tree seedlings in the nursery and field; 5) characterise AMF for nursery application; 6) investigate ecological roles, e.g. whether heat resistant AMF colonise heat resistant bracken fern. The succession of AMF will be monitored by 454FLX Titanium deep sequencing, and compared to natural forest data. The identification of AMF useful for efficient reforestation attempts will help to speed-up the slow succession towards a natural , diverse forest.

所描述的215种球囊菌门仅代表其物种多样性的一小部分。目前对丛枝菌根真菌（AMF，Glomeromycota）的分子生态学群落分析大多缺乏物种水平的分辨率，且所用方法往往存在较大的偏倚。我们正在引入改进的方法来解决分子AMF监测的缺陷。为了了解专性共生AMF在厄瓜多尔本土树木中的功能作用，并使AMF适用于造林，我们将：1）进行天然林幼苗AMF的分子监测（基线数据）; 2）确定在>4年的地块中先前种植的树木中建立的AMF（基线数据）; 3）监测AMF在直播树苗中的演替（4）评价接种丛枝菌根真菌对提高苗圃和田间苗木生长性能的潜力;（5）研究AMF在苗圃中的应用;（6）研究AMF的生态学作用，如耐热AMF是否能在耐热蕨菜中定植。AMF的演替将通过454 FLX Titanium深度测序进行监测，并与天然林数据进行比较。AMF的识别有助于有效的重新造林尝试将有助于加快缓慢的演替，走向自然，多样性的森林。