Properties and functions of plant CDP-diacylglycerol synthases

植物CDP-二酰基甘油合酶的特性和功能

• 批准号: 36024888
• 负责人: Professorin Dr. Margrit Frentzen
• 金额: --
• 依托单位: AG Frentzen (Spezielle Botanik)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/36024888?language=en
• 关键词: Properties; functions; plant; CDP; diacylglycerol

Cytidine-diphosphate-diacylglycerol (CDP-DAG) synthases catalyse the activation of phosphatidic acid to CDP-DAG, a central intermediate in glycerolipid biosynthesis of both prokaryotic and eukaryotic organisms. It is the direct precursor to phosphatidylinositol, -serine, -glycerol and cardiolipin in plant cells which are essential for various cellular functions. Studies with yeast and insect cells provided evidence that CDP-DAG is involved in regulating glycerolipid biosynthesis and lipid dependent signal transduction processes. In plants, CDP-DAG synthases are located in plastids, mitochondria and the endomembrane system, but apart from their subcellular localisation very little is known about these isozymes. To gain access to this important protein class and to study their functions in plants, the genes of Arabidopsis thaliana encoding CDP-diacylglycerol synthases are to be functionally characterised with regard to the properties of the encoded proteins and their targeting within plant cells. These experiments shall be complemented by the development and analysis of A. thaliana mutants and transgenic lines, deficient in certain CDP-diacylglycerol synthases or over-expressing the respective ones in order to investigate their functional roles.

胞苷二磷酸二酰基甘油（CDP-DAG）酶催化磷脂酸活化为CDP-DAG，CDP-DAG是原核生物和真核生物的甘油脂质生物合成的中心中间体。它是植物细胞中磷脂酰肌醇、β-丝氨酸、β-甘油和心磷脂的直接前体，对各种细胞功能至关重要。用酵母和昆虫细胞的研究提供了CDP-DAG参与调节甘油脂质生物合成和脂质依赖性信号转导过程的证据。在植物中，CDP-DAG同工酶位于质体、线粒体和内膜系统中，但除了它们的亚细胞定位之外，对这些同工酶知之甚少。为了获得这类重要的蛋白质并研究它们在植物中的功能，将对编码CDP-二酰基甘油脱氢酶的拟南芥基因的编码蛋白质的性质及其在植物细胞内的靶向进行功能表征。这些实验将通过开发和分析A.拟南芥突变体和转基因株系，缺乏某些CDP-二酰基甘油脱氢酶或过度表达各自的，以研究其功能作用。