Deciphering the Functions of Gustatory Receptors In the Recognition of Host Plant and Midgut Environment Using Knockout Silkworms

利用基因敲除蚕破译味觉受体在识别宿主植物和中肠环境中的功能

• 批准号: 21K14859
• 负责人: 忙 定沢
• 金额: $ 2.91万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K14859/
• 关键词: Gustatory receptor; Peptide hormone; Enteroendocrine cells; Neurosecretory cells; Tachykinin; Gustatory Receptors; host-plant recognition; midgut environment; peptide hormone

We first used RT-PCR to investigate expression of BmGr4 gene, a member of the putative sugar clade gene family in various tissues. BmGr4 is expressed in the midgut and central nervous system (CNS). Moreover, immunohistochemistry using an anti-BmGr4 anti-serum demonstrated that BmGr4 is expressed in cells by midgut and nervous system. Furthermore, double-immunohistochemistry indicated that BmGr4 is expressed in midgut enteroendocrine cells, also in CNS neurosecretory cells. In particular, a portion of BmGr4-expressing cells, in both midgut and CNS, secretes tachykinin peptides. These results suggest that BmGr4 functions not only as a taste receptor, but also as a chemical sensor such as for the regulation of gut movement, physiological conditions, and feeding behavior of larvae.

我们首先使用RT-PCR技术研究了BmGr4基因在不同组织中的表达，BmGr4基因是糖枝基因家族的一员。BmGr4在中肠和中枢神经系统（CNS）表达。此外，使用抗BmGr4抗血清的免疫组织化学表明，BmGr4在细胞中通过中肠和神经系统表达。双免疫组化结果表明，BmGr4在中肠肠内分泌细胞和中枢神经系统分泌细胞中均有表达。特别是，在中肠和中枢神经系统中，部分表达bmgr4的细胞分泌速激肽。这些结果表明，BmGr4不仅具有味觉感受器的功能，还具有调节肠道运动、生理状况和幼虫摄食行为的化学传感器功能。