Proteomics and peptidomics for the identification and functional characterization of sORF encoded proteins

与第一个资助期一样，蛋白质组学和多肽Z项目旨在以合作项目的形式，为SPP-2002中那些没有自己的内部蛋白质组学议程作为主要任务的小组提供最先进的分析方法。根据第一个资助期的需求和申请者为第二个资助期设想的研究计划，将解决四项主要任务：(I)SEP的鉴定和量化；(Ii)比较蛋白质组表达分析，以破译SEP的功能潜力；(Iii)SEP相互作用伙伴的确定；以及(Iv)翻译后修饰的SEP的特征。尽管在SEP分析领域取得了显著进展，包括这一Z项目成员在第一个资助期所做的方法学工作，但仍然需要改进和全新的分析方法。因此，作为第二项任务，Z项目将继续开展以方法学为导向的研究，以便(I)改进现有方法，使其适应项目合作伙伴的具体要求，(Ii)能够提供新的、未经测试的技术，(Iii)将新方法直接转让给合作项目，以取得更好的科学成果，(Iv)在这一国际知名度很高的研究领域以及作为第一个资助期的必要生物分析领域设定基准。主要焦点将集中在SEP的量化方法、小蛋白翻译后修饰的分析以及SEP的自上而下分析上。

As in the first funding period, the proteomics and peptidomics Z-project aims to provide, in form of cooperation projects, state-of-the-art analytical approaches for those groups in the SPP-2002 not having own in-house proteomics agendas as the primary task. Following the demands from the first funding period and the research programs envisaged by the applicants for the second funding period, four main tasks will be addressed: (i) the identification and quantification of SEP; (ii) comparative proteome expression analysis in order to decipher the functional potential of SEP; (iii) the identification of SEP interaction partners; and (iv) the characterization of posttranslational modified SEP. Despite remarkable advances made in the field of analytics of SEP, including the methodological work contributed in the first funding period by the members of this Z-project, there is still an ongoing need for improved and completely new analytical approaches. Consequently, as a second task the Z-project will continue to perform methodological oriented research in order to (i) improve existing approaches and adapt these to the projects partners specific demands, (ii) to be capable to provide novel, yet untested technologies, (iii) to directly transfer novel methods to cooperation projects for an enhanced scientific outcome and (iv) to set benchmarks in this internationally highly visible research field as well as in the field of the necessary bioanalytics as for the first funding period. Major foci will lie on approaches for the quantification of SEP, the analysis of posttranslational modifications in the small proteins and on the top-down analysis of SEP.