Proteomics and peptidomics for the identification and functional characterization of sORF encoded proteins

用于 sORF 编码蛋白的鉴定和功能表征的蛋白质组学和肽组学

• 批准号: 378209894
• 负责人: Professorin Dr. Dörte Becher
• 金额: --
• 依托单位: Arbeitsgruppe Systemische Proteomics und Bioanalytik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/378209894?language=en
• 关键词: Proteomics; peptidomics; identification; functional; characterization

As in the first funding period, the proteomics and peptidomics Z-project aims to provide, in form of cooperation projects, state-of-the-art analytical approaches for those groups in the SPP-2002 not having own in-house proteomics agendas as the primary task. Following the demands from the first funding period and the research programs envisaged by the applicants for the second funding period, four main tasks will be addressed: (i) the identification and quantification of SEP; (ii) comparative proteome expression analysis in order to decipher the functional potential of SEP; (iii) the identification of SEP interaction partners; and (iv) the characterization of posttranslational modified SEP. Despite remarkable advances made in the field of analytics of SEP, including the methodological work contributed in the first funding period by the members of this Z-project, there is still an ongoing need for improved and completely new analytical approaches. Consequently, as a second task the Z-project will continue to perform methodological oriented research in order to (i) improve existing approaches and adapt these to the projects partners specific demands, (ii) to be capable to provide novel, yet untested technologies, (iii) to directly transfer novel methods to cooperation projects for an enhanced scientific outcome and (iv) to set benchmarks in this internationally highly visible research field as well as in the field of the necessary bioanalytics as for the first funding period. Major foci will lie on approaches for the quantification of SEP, the analysis of posttranslational modifications in the small proteins and on the top-down analysis of SEP.

与第一个资助期一样，蛋白质组学和肽组学Z项目旨在以合作项目的形式，为SPP-2002中没有自己的内部蛋白质组学议程作为主要任务的小组提供最先进的分析方法。根据第一个资助期的需求和申请人在第二个资助期设想的研究计划，将进行四项主要工作：（i）SEP的鉴定和定量;（ii）比较蛋白质组表达分析，以破译SEP的功能潜力;（iii）SEP相互作用伙伴的鉴定;（iv）SEP的基因表达。和（iv）翻译后修饰SEP的表征。尽管SEP分析领域取得了显著进展，包括本Z项目成员在第一个资助期内贡献的方法学工作，仍然需要改进的和全新的分析方法。因此，作为第二项任务，Z-项目将继续进行方法导向的研究，以便（i）改进现有方法并使其适应项目合作伙伴的具体需求，（ii）能够提供新颖但未经测试的技术，（三）直接将新方法转让给合作项目，以提高科学成果;在这一国际知名度很高的研究领域以及必要的生物分析领域，为第一个资助期设定基准。主要焦点将在于定量SEP的方法，小蛋白的翻译后修饰的分析和自上而下的SEP分析。