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Microfluidic flow in printed fracture channel

打印裂缝通道中的微流体流动

基本信息

批准号：
19F19329
负责人：
Sivaniah Easan
金额：
$ 1.41万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2019
资助国家：
日本
起止时间：
2019-10-11 至 2022-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19329/
关键词：
Paper submitted Water flow in OM film Electrophoresis OM films on aluminium Large image on OM film Maskless printing of OM Expanded film size of OM Microfluidic flow in OM Improved OM on plastic Shared OM with community

项目摘要

In the final stages of the project, ending 2021, I began a collaboration with a Genetics research group to help develop the Organized Microfibrillation process for microfluidics. Up until this point I had difficulty getting water based solutions into the porous OM polymer films. Minor success was achieved with common additives and surfactants. With this new collaboration we were successfully able to identify some chemicals that enabled the flow of water based solutions in my OM channels. With this breakthrough, we were able to achieve other milestones with the OM films. Relevant biological materials, such as proteins and saccharides could enter the channels and could be tracked by fluorescent microscope. Finally we were able to achieve separation of biological materials with differing molecular weights, including separation of proteins and saccharaides, and separation of insulin and inert SARS-COV2 capsid. Separation was achieved by making OM channels with differing porosity such that larger molecules were blocked by the pores. These achievements have been included in a paper that was submitted to Nature Communications.With the same collaborators I also worked on how to move liquids through OM films without capillary action. A weakness of OM for microfluidics is the lack of pumping capability. During this time I tried applying electrophoresis to my OM films. I and my collaborators explored different experimental setups and conditions and were able to achieve electrophoretic flow preliminary experiments.

在项目的最后阶段，到2021年底，我开始与遗传学研究小组合作，帮助开发微流体的有组织微纤化过程。在此之前，我很难将水基溶液引入多孔OM聚合物膜中。使用普通添加剂和表面活性剂取得了较小的成功。通过这次新的合作，我们成功地确定了一些化学品，使我的OM渠道中的水基解决方案能够流动。通过这一突破，我们能够在OM薄膜方面实现其他里程碑。相关的生物物质，如蛋白质和蛋白质可以进入通道，并可以通过荧光显微镜跟踪。最后，我们能够实现具有不同分子量的生物材料的分离，包括蛋白质和糖类的分离，以及胰岛素和惰性SARS-COV 2衣壳的分离。通过制造具有不同孔隙率的OM通道来实现分离，使得较大的分子被孔阻挡。这些成果已被收录在一篇提交给《自然通讯》的论文中。我还与同样的合作者一起研究了如何在没有毛细作用的情况下使液体通过OM薄膜。用于微流体的OM的弱点是缺乏泵送能力。在此期间，我尝试将电泳应用于我的OM薄膜。我和我的合作者探索了不同的实验设置和条件，并能够实现电泳流动的初步实验。