Low-power energy, portable nano-sensor chipset devices as early-stage diagnoses of neuronal diseases

通过第二年，我们研究了用于监测活细胞分泌的神经递质（SHSY-5Y和PC12）的芯片组生物传感器设计。采用金光刻技术制备芯片组生物传感器，形成交叉指状电极阵列（IDA）。在小样本量和低功耗条件下，该IDA具有良好的性能和电化学活性。所设计的杂原子掺杂碳材料（N掺杂碳和P掺杂碳）表现出低细胞毒性和高相容性的高稳定性材料。所设计的IDA电极被合成的碳材料修饰，用于稳定神经递质芯片组纳米生物传感器，具有较高的稳定性和重复性。所设计的芯片组纳米生物传感器能够灵敏、选择性地检测人血清和SHSY-5Y和PC12神经元细胞系模型中多巴胺、肾上腺素、去甲肾上腺素和乙酰胆碱。所设计的芯片组纳米生物传感器具有信号稳定性高、检出限低、功耗低、经济实惠、现场检测快（5 ~ 10 s）、样品体积小（10uL）等特点。因此，我们成功地设计了一种便携式芯片组纳米生物传感器，用于敏感和选择性地监测神经递质资源和受体。此外，这些设计的便携式纳米生物传感器可用于神经疾病的随访和诊断，如精神分裂症、阿尔茨海默病和帕金森病。

Through the second year, we studied the chipset biosensor design for monitoring of neurotransmitters secreted from living cells (SHSY-5Y and PC12). The chipset biosensor was fabricated using photolithography using gold and formed interdigitated electrode array (IDA). The IDA showed high performance and high electrochemical activity at low sample volume and low power consumption. The designed materials of heteroatoms doped carbon (N- and P- doped carbon) displayed a highly stable materials with low cytotoxicity and high compatibility. The designed IDA electrode was modified by the synthesized carbon materials for stabilising the neurotransmitters chipset nanobiosensor with high stability and reproducibility. The designed chipset nanobiosensors indicated a sensitive and selective assay for detection of dopamine, adrenaline, noradrenaline, and acetylcholine from various resources such as human blood serum and neuronal cell line models of SHSY-5Y and PC12. The designed chipset nanobiosensors exhibited high signal stability, low detection limit, low power consumption, economical value, on-site detection, fast response (5-10 s), and low sample volume (10uL). Therefore, we successfully designed a portable chipset nanobiosensor for sensitive and selective monitoring of neurotransmitters in their resources and receptors. Furthermore, theses designed portable nanobiosensors can be used for follow -up and diagnosis of neuronal disorders such as schizophrenia, Alzheimer, and Parkinson’s diseases.