Low-power energy, portable nano-sensor chipset devices as early-stage diagnoses of neuronal diseases

第二年，我们研究了用于监测活细胞分泌的神经递质的芯片组生物传感器设计（SHSY-5 Y和PC 12）。芯片生物传感器的制作使用光刻使用金和形成叉指电极阵列（IDA）。IDA在低样品体积和低功耗下表现出高性能和高电化学活性。所设计的杂原子掺杂碳（N-和P-掺杂碳）材料表现出高稳定性、低细胞毒性和高相容性。所设计的IDA电极被合成的碳材料修饰，用于稳定具有高稳定性和重现性的神经递质芯片组纳米生物传感器。所设计的芯片组纳米生物传感器指示用于检测来自各种资源（例如人类血清和SHSY-5 Y和PC 12的神经元细胞系模型）的多巴胺、肾上腺素、去甲肾上腺素和乙酰胆碱的灵敏和选择性测定。所设计的芯片型纳米生物传感器具有信号稳定性高、检测限低、功耗低、经济实用、现场检测、响应速度快（5-10 s）、样品体积小（10 uL）等特点。因此，我们成功地设计了一种便携式芯片组纳米生物传感器，用于敏感和选择性地监测其资源和受体中的神经递质。此外，这些设计的便携式纳米生物传感器可用于跟踪和诊断神经元疾病，如精神分裂症，阿尔茨海默病和帕金森病。

Through the second year, we studied the chipset biosensor design for monitoring of neurotransmitters secreted from living cells (SHSY-5Y and PC12). The chipset biosensor was fabricated using photolithography using gold and formed interdigitated electrode array (IDA). The IDA showed high performance and high electrochemical activity at low sample volume and low power consumption. The designed materials of heteroatoms doped carbon (N- and P- doped carbon) displayed a highly stable materials with low cytotoxicity and high compatibility. The designed IDA electrode was modified by the synthesized carbon materials for stabilising the neurotransmitters chipset nanobiosensor with high stability and reproducibility. The designed chipset nanobiosensors indicated a sensitive and selective assay for detection of dopamine, adrenaline, noradrenaline, and acetylcholine from various resources such as human blood serum and neuronal cell line models of SHSY-5Y and PC12. The designed chipset nanobiosensors exhibited high signal stability, low detection limit, low power consumption, economical value, on-site detection, fast response (5-10 s), and low sample volume (10uL). Therefore, we successfully designed a portable chipset nanobiosensor for sensitive and selective monitoring of neurotransmitters in their resources and receptors. Furthermore, theses designed portable nanobiosensors can be used for follow -up and diagnosis of neuronal disorders such as schizophrenia, Alzheimer, and Parkinson’s diseases.