Low-power energy, portable nano-sensor chipset devices as early-stage diagnoses of neuronal diseases

第二年，我们研究了用于监测活细胞(SHSY-5Y和PC12)分泌的神经递质的芯片组生物传感器设计。利用金的光刻技术制作了芯片组生物传感器，并形成了交叉型电极阵列。IDA在小样品量、低功耗下表现出高性能和高电化学活性。所设计的杂原子掺杂碳(氮、磷掺杂碳)材料表现出高度稳定、低细胞毒性和高相容性。设计的IDA电极被合成的碳材料修饰，以稳定神经递质芯片组纳米生物传感器，具有高的稳定性和重复性。设计的芯片组纳米生物传感器显示了一种灵敏和选择性的检测方法，可以检测来自不同来源的多巴胺、肾上腺素、去甲肾上腺素和乙酰胆碱，例如人血清和神经细胞模型SHSY-5Y和PC12。所设计的芯片组纳米生物传感器具有信号稳定性高、检测下限低、功耗低、经济实惠、现场检测、响应快(S 5~10)、样本量小(10 UL)等特点。因此，我们成功地设计了一种便携式芯片组纳米生物传感器，用于灵敏和选择性地监测神经递质的来源和受体。此外，这些设计的便携式纳米生物传感器可以用于精神分裂症、阿尔茨海默病和帕金森病等神经元疾病的跟踪和诊断。

Through the second year, we studied the chipset biosensor design for monitoring of neurotransmitters secreted from living cells (SHSY-5Y and PC12). The chipset biosensor was fabricated using photolithography using gold and formed interdigitated electrode array (IDA). The IDA showed high performance and high electrochemical activity at low sample volume and low power consumption. The designed materials of heteroatoms doped carbon (N- and P- doped carbon) displayed a highly stable materials with low cytotoxicity and high compatibility. The designed IDA electrode was modified by the synthesized carbon materials for stabilising the neurotransmitters chipset nanobiosensor with high stability and reproducibility. The designed chipset nanobiosensors indicated a sensitive and selective assay for detection of dopamine, adrenaline, noradrenaline, and acetylcholine from various resources such as human blood serum and neuronal cell line models of SHSY-5Y and PC12. The designed chipset nanobiosensors exhibited high signal stability, low detection limit, low power consumption, economical value, on-site detection, fast response (5-10 s), and low sample volume (10uL). Therefore, we successfully designed a portable chipset nanobiosensor for sensitive and selective monitoring of neurotransmitters in their resources and receptors. Furthermore, theses designed portable nanobiosensors can be used for follow -up and diagnosis of neuronal disorders such as schizophrenia, Alzheimer, and Parkinson’s diseases.