非天然型アミノ酸生産のためのPLP依存性アミノ基転移酵素の改変

修饰 PLP 依赖性转氨酶以产生非天然氨基酸

• 批准号: 19F19764
• 负责人: LAURINO Paola
• 金额: $ 1.34万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2019
• 资助国家: 日本
• 起止时间: 2019-11-08 至 2022-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-19F19764/
• 关键词: Phase separation; enzymes; enzyme kinetic; protein engineering; protein plasticity; Disordered regions; AGT; protocells; Liquid phase separation; Biochemistry; enzyme; library; screening

・The production/generation of liquid-liquid phase separated membrane-less droplets with novel features as the ability to compartmentalize enzymes efficiently, high protein crowding (mM protein content) to study in detail the effects of crowding on proteins and especially on the enzymatic activity (using length scale of living systems micrometer scale as cells).・We also demonstrated how the enzymatic activity in presence of crowding generated flow similar to the cytoplasmatic streaming of the cell. This work has been published by Nature Communications it is amongst the 2021 top 25 most downloaded Chemistry and Material Science Articles in 2021 published in Nature communication.・In addition, we have discovered how protein plasticity shape the function and fitness. The work has been published in Protein Science.

·生产/产生液-液相分离的无膜液滴，其具有新的特征，如有效区室化酶的能力，高蛋白质拥挤（mM蛋白质含量），以详细研究拥挤对蛋白质的影响，特别是对酶活性的影响（使用生命系统微米尺度的长度尺度作为细胞）。我们还证明了在拥挤的存在下，酶活性如何产生类似于细胞的胞质流动的流动。这项工作已发表在Nature Communications上，它是2021年2021年下载量最高的25篇化学和材料科学文章之一。此外，我们还发现了蛋白质可塑性如何塑造功能和适应性。这项研究成果发表在Protein Science上。

项目成果

Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors

• DOI: 10.1016/j.bios.2020.112419
• 发表时间: 2020-10-01
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Danielson, Eric;Dindo, Mirco;Sowwan, Mukhles
• 通讯作者: Sowwan, Mukhles

Cycloserine enantiomers are reversible inhibitors of human alanine:glyoxylate aminotransferase: implications for Primary Hyperoxaluria type 1

• DOI: 10.1042/bcj20190507
• 发表时间: 2019-12-01
• 期刊: BIOCHEMICAL JOURNAL
• 影响因子: 4.1
• 作者: Dindo, Mirco;Grottelli, Silvia;Cellini, Barbara
• 通讯作者: Cellini, Barbara