Correlative cryo-light microscope (cryo-LM)

相关冷冻光显微镜 (cryo-LM)

• 批准号: 384149399
• 金额: --
• 依托单位: Charité - Universitätsmedizin Berlin
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/384149399?language=en
• 关键词: Correlative; cryo; light; microscope; LM

In order to prepare specimen suited for high-resolution cryo-ET, additional instruments are required. The requested correlative cryo-light microscope (cryo-LM) shall facilitate cryo-fluorescence imaging in a correlated manner with electron microscopy on the 300 kV cryo-TEM for tomography and FIB-milling / scanning electron microscopy on the dual beam cryo-FIBSEM instrument. The main purpose of the cryo-LM is thus to guide the selection of targets for cryo-ET via fluorescent labels (e.g. genetically encoded GFP). Furthermore, the instrument will be used for correlative light/electron microscopic (CLEM) applications . The instrument shall be integrated into the Charité EM core facility to facilitate the cryo-ET workflow, but at the same time provide an interface to the Charité Advanced Medical Bioimaging Core Facility (AMBIO).

为了制备适合于高分辨率冷冻的样品，需要额外的仪器。所要求的相关低温光学显微镜(CRYO-LM)应以与300千伏低温电子显微镜上的电子显微镜相关的方式促进低温荧光成像，以便在双光束CRYO-FIBSEM仪器上进行FIB-MILING/扫描电子显微镜。因此，CRYO-LM的主要目的是通过荧光标记(例如，基因编码的GFP)来指导选择CRYO-ET的目标。此外，该仪器还将用于相关的光学/电子显微镜(CLEM)应用。该仪器应集成到CharitéEM核心设施中，以促进冷冻工作流程，但同时提供与CharitéAdvanced医学生物成像核心设施(AMBIO)的接口。