Molecular manipulation of Arabidopsis by type III effectors from the bacterial plant pathogens Pseudomonas syringae and Xanthomonas

细菌植物病原体丁香假单胞菌和黄单胞菌的 III 型效应子对拟南芥的分子操作

• 批准号: 38417143
• 负责人: Professor Dr. Jens Boch
• 金额: --
• 依托单位: Institut für Pflanzengenetik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/38417143?language=en
• 关键词: Molecular; manipulation; Arabidopsis; type; III

Plant pathogenic Pseudomonas syringae and Xanthomonas spp. reprogram plant cells with effector proteins that are translocated into the plant cell cytoplasm via a type III secretion system. We study the molecular activities of effectors and aim to identify their plant targets. Subcellular localization studies of P. syringae effectors with putative ADP-ribosyltransferase (ADP-RT) activity suggests that they target plant components at the plasma membrane and the microtubular network. We will identify plant targets using interaction screens and enzyme assays. TAL effectors from Xanthomonas bind specific promoter DNA sequences of plant genes and induce their expression. The DNA binding is mediated by a novel, modular DNA binding domain which determines a predictable specificity. TALs from Arabidopsis-pathogenic Xanthomonas and artificial TALs will be studied to understand the molecular parameters for specificity of action.

植物病原性假单胞菌和黄单胞菌。用效应蛋白重编程植物细胞，所述效应蛋白通过III型分泌系统易位到植物细胞质中。我们研究效应子的分子活性，目的是确定它们的植物靶标。具有推定的ADP-核糖基转移酶（ADP-RT）活性的P. longingae效应子的亚细胞定位研究表明，它们靶向质膜和微管网络处的植物组分。我们将使用相互作用筛选和酶测定来识别植物靶点。来自黄单胞菌属的TAL效应子结合植物基因的特异性启动子DNA序列并诱导其表达。DNA结合是由一个新的，模块化的DNA结合结构域，确定了可预测的特异性介导。将研究来自黄单胞菌属致病性黄单胞菌的TAL和人工TAL以了解作用特异性的分子参数。