The role of glycolipids at the interface of plant-microbe interactions during nodulation and myorrhiza formation in Lotus japonicus

糖脂在百脉根结瘤和菌根形成过程中植物-微生物相互作用界面中的作用

• 批准号: 38478495
• 负责人: Professor Dr. Peter Dörmann
• 金额: --
• 依托单位: Institut für Molekulare Physiologie und Biotechnologie der Pflanzen (IMBIO)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/38478495?language=en
• 关键词: role; glycolipids; interface; plant; microbe

Glycolipids are constituents of membranes at the boundary between plants and microorganisms. We are studying the role of galactoglycerolipids, sterol glucosides and glucosylceramides in the symbiotic interaction of Lotus japonicus plants with Mesorhizobium during nodulation or with the fungus Glomus during mycorrhiza formation. Because each glycolipid class consists of numerous molecular species, a quadrupole-time of flight mass spectrometry (Q-TOF MS) based method was developed for the measurement of glycoglycerolipids, sterol lipids and sphingolipids. This includes the first development of a method for the measurement of all sterol lipid classes in plants (free sterols, sterol glucosides, sterol esters, acylated sterol glucosides). Transgenic Lotus plants carrying RNAi constructs for the genes involved in the synthesis of glycolipids (DGD1, DGD2, SGT1, SGT2, GCS) were generated during the previous funding period. Lipid measurements by Q-TOF MS revealed that sterol glucosides and glucosylceramides are reduced in SGT2 and GCS RNAi lines, respectively. We obtained TILLING mutants for the Lotus genes DGD1, DGD2, SGT2 and GCS. Nodulation and mycorrhization experiments of the transgenic RNAi lines and of the mutant lines are underway. Furthermore, we developed a non-targeted Q-TOF MS approach that we will employ to search for lipids responding to nodulation or mycorrhization in Lotus.

糖脂是植物和微生物之间边界膜的成分。我们正在研究半乳糖甘油脂、甾醇糖苷和葡萄糖神经酰胺在百脉根植物与中生根瘤菌在结瘤过程中或在菌根形成过程中与真菌球囊菌的共生相互作用中的作用。由于每一类糖脂均由众多分子种类组成，因此开发了基于四极杆飞行时间质谱 (Q-TOF MS) 的方法来测量甘油糖脂、甾醇脂质和鞘脂。这包括首次开发一种测量植物中所有甾醇脂质类别（游离甾醇、甾醇糖苷、甾醇酯、酰化甾醇糖苷）的方法。携带参与糖脂合成的基因（DGD1、DGD2、SGT1、SGT2、GCS）的 RNAi 构建体的转基因莲花植物是在上一个资助期间产生的。 Q-TOF MS 的脂质测量表明，SGT2 和 GCS RNAi 系中的甾醇葡萄糖苷和葡萄糖神经酰胺分别减少。我们获得了 Lotus 基因 DGD1、DGD2、SGT2 和 GCS 的 TILLING 突变体。转基因RNAi品系和突变品系的结瘤和菌根化实验正在进行中。此外，我们开发了一种非靶向 Q-TOF MS 方法，我们将利用该方法来寻找对莲花中结瘤或菌根化有反应的脂质。