Maintenance of chromatin organization by Isw1a during gene transcription (A19*)

我们正在研究Isw1a重塑子在维持核小体组织中的作用，通过回收现有的组蛋白，并防止与乙酰化水平增加相关的新的可溶性组蛋白的掺入。通过限制组蛋白动力学和乙酰化组蛋白的掺入，Isw1a被假设可以防止不适当的转录起始和非编码rna的产生。我们将使用酵母遗传学、基因组学、生物化学和蛋白质组学等组合方法来研究Isw1a功能的分子机制，包括Isw1a的招募和保留。

We are investigating the role of the Isw1a remodeler in maintaining nucleosomal organization over genes by recycling existing histones and preventing the incorporation of new, soluble histones associated with increased acetylation levels. By limiting histone dynamics and the incorporation of acetylated histones Isw1a is hypothesized to prevent inappropriate transcription initiation and the production of non-coding RNAs. We will use a combinatorial approach including yeast genetics, genomics, biochemistry and proteomics to investigate the molecular mechanisms underlying Isw1a function, including Isw1a recruitment and retention.