Identification and characterization of thiol switches controlling the yeast metabolic cycle

[1]阐明酵母菌代谢周期中H_2O_2变化的时空动态。[2]研究YMC过程中硫醇过氧化物酶相互作用组的变化。(I)利用氧化还原蛋白质组学来寻找YMC过程中蛋白质硫醇氧化的变化(Ii)评估硫醇过氧化物酶对蛋白质硫醇氧化的重要性(Iii)采用亲和纯化和邻近相关的生物素化方法直接鉴定硫醇过氧化物酶相互作用伙伴，并确定相互作用伙伴的保留范围是否在YMC的不同阶段发生变化。[3]对有希望的候选硫醇开关进行生化表征，以从机制上确定它们如何影响YMC

[1] Elucidate the spatio-temporal dynamics of H2O2 changes during the yeast metabolic cycle.[2] Investigate changes in the thiol peroxidase interactome during the YMC.(i) Use redox proteomics to look for changes in protein thiol oxidation during the YMC(ii) Assess the importance of thiol peroxidases for (changes in) protein thiol oxidation(iii) Employ affinity purification and proximity-associated biotinylation approaches to directly identify thiol peroxidase interaction partners and determine whether the repertoire of interaction partners changes at different stages of the YMC. [3] Biochemically characterize promising candidate thiol switches to determine mechanistically how they affect the YMC