Development of New Bioorthogonal Reactions Based on Rational Computational Design

基于理性计算设计的新型生物正交反应的开发

• 批准号: 386374799
• 负责人: Dr. Michal Majek
• 金额: --
• 依托单位: Institut für Chemie
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/386374799?language=en
• 关键词: Development; New; Bioorthogonal; Reactions; Based

Bioorthogonal chemistry makes in situ modification of biomacromolecules possible, opening avenues to study cellular processes without disruption of the living organisms. This interdisciplinary project combines theoretical, synthetic and mechanistic research work to develop new bioorthogonal reactions. It will result in the development of a new set of reagents designed for use in later biological studies. A major obstacle of in vivo applications of bioorthogonal reactions is the limited robustness of the employed reaction partners in the presence of biogenic nucleophiles. In this project, we aim to discover a combination of computational and spectroscopic methods to predict the stability of the reagents that are commonly used in bioorthogonal reactions. Special attention will be given to the development of visible light-induced bioorthogonal reactions, as not much progress was made in this field so far, and such reactions are of utmost interest for the selective labeling of biomacromolecules.

生物正交化学使生物大分子的原位修饰成为可能，为研究细胞过程而不破坏活生物体开辟了途径。这个跨学科的项目结合了理论，合成和机理研究工作，以开发新的生物正交反应。这将导致开发一套新的试剂，用于以后的生物学研究。生物正交反应在体内应用的一个主要障碍是在生物亲核试剂的存在下所采用的反应伴侣的有限稳健性。在这个项目中，我们的目标是发现计算和光谱方法的组合，以预测生物正交反应中常用的试剂的稳定性。特别注意将给予可见光诱导的生物正交反应的发展，因为到目前为止在这一领域取得的进展不大，这样的反应是最感兴趣的生物大分子的选择性标记。