Mechanisms of parasitic RNA propagation and elimination in male germline studied on economically important viroid species

高等植物的单倍体雄性生殖系通过雄配子的产生和运输、双受精、种子生产以及最终通过有效的消除花粉中的寄生核酸的机制在植物育性和作物生产中起着至关重要的作用。一些类病毒--适合逃避这种沉默机制的寄生性单链环状RNA--可以通过花粉传播（通过花粉传播）或从发育中的花粉中消除，具体取决于植物-病原体组合。目前还没有关于花粉中类病毒分子相互作用的信息，但可以假设类病毒RNA与发育中的雄配子体的代谢特异性相互作用，涉及小RNA。在这里，我们将使用一个啤酒花分离的苹果果实皱纹类病毒感染的烟草花粉研究模型，分析转录组，降解组和蛋白质组数据的识别机制（S）抑制类病毒的传播，并导致类病毒消除在确定的检查点在雄性生殖系发育。主要目的是表征花粉发育过程中寄生类病毒RNA与烟草基因表达的相互作用，并确定与类病毒抑制和雄性生殖系消除相关的自然机制。

The haploid male germline of higher plants plays a vital role in plant fertility and crop production through the generation and transport of male gametes, double fertilization, seed production and finally through effective mechanisms of the elimination of parasitic nucleic acids from pollen. Some viroids---parasitic single-stranded circular RNAs adapted to escape such silencing mechanism---can be either pollen-transmissible (spread via pollen) or eliminated from developing pollen, depending on plant-pathogen combinations. There is currently no information about molecular interaction of viroids within pollen but it can be assumed that viroid RNA interacts specifically with the metabolism of developing male gametophyte involving small RNAs. Here we will use a hop isolate of apple fruit crinkle viroid infection of Nicotiana tabacum as a pollen research model to analyze transcriptomic, degradomic and proteomic data for the identification of mechanism(s) suppressing viroid-propagation and leading to viroid elimination at defined check points during male germline development. The main objective is to characterize the interaction of parasitic viroid RNA with tobacco gene expression during pollen development and to identify the natural mechanism(s) associated with viroid suppression and elimination from the male germline.