Understanding Microbial Rhodopsins by linking Solid-State NMR with computational Approaches

通过将固态核磁共振与计算方法联系起来了解微生物视紫红质

• 批准号: 391643887
• 负责人: Professor Dr. Clemens Glaubitz
• 金额: --
• 依托单位: National Natural Science Foundation of China
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/391643887?language=en
• 关键词: Understanding; Microbial; Rhodopsins; linking; Solid

Membrane proteins of the proteorhodopsin (PR) family are the most abundant retinal-based photoreceptors on this planet. Their function as light-driven proton pump creates a significant contribution to the bioenergetics in marine ecosystems. In addition, they could fulfill an array of additional sensing and regulatory activities. In this project, we will resolve the molecular details of color tuning between green and blue PR as well as red-absorbing mutants at atomic resolution, elucidate structural and electronic changes during the photocycle and dissect the functional role of specific cross-protomer interactions within the native pentamers. This project combines solid-state NMR including dynamic nuclear polarization and light-induced cryo-trapping in the Glaubitz lab, Frankfurt, with QM/MM-based calculations of NMR parameters, absorption spectra and MD simulations in the He lab, Shanghai. In this way, we will be able to connect optical properties with structural and electronic parameters. Such a concerted application of experimental and theoretical approaches will also result in protocols and procedures, which could be applied to other membrane proteins in the future.

蛋白视紫质（PR）家族的膜蛋白是这个星球上最丰富的视网膜光感受器。它们作为光驱动质子泵的功能为海洋生态系统的生物能量学做出了重大贡献。此外，它们还可以完成一系列额外的传感和调节活动。在这个项目中，我们将解决绿色和蓝色PR之间的颜色调谐以及红色吸收突变体在原子分辨率的分子细节，阐明结构和电子的光循环过程中的变化和解剖的功能作用，具体的跨原生五聚体的相互作用。该项目结合了法兰克福Glaubitz实验室的固态NMR（包括动态核极化和光诱导低温捕获）和上海He实验室的基于QM/MM的NMR参数计算、吸收光谱和MD模拟。通过这种方式，我们将能够将光学性质与结构和电子参数联系起来。这种实验和理论方法的协调应用也将导致协议和程序，这可以应用于其他膜蛋白在未来。