Study of Bacterial Biofilms by Surface-enhanced Raman Microscopy

表面增强拉曼显微镜研究细菌生物膜

• 批准号: 392287292
• 负责人: Professor Dr. Jürgen Popp
• 金额: --
• 依托单位: Forschungsabteilung Spektroskopie / Bildgebung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/392287292?language=en
• 关键词: Study; Bacterial; Biofilms; Surface; enhanced

Biofilms present a universal way of microbial life in a natural environment. They can grow on nearly every surface in contact with water. Besides cellular constituents, extracellular polymeric substances (EPS) are one major fraction of the biofilm matrix. The detailed knowledge about the chemical distributions of the EPS matrix is relevant e.g. for the optimization of biocides, of antifouling strategies and for biological wastewater treatment. A powerful approach to non-destructively investigate the detailed chemical information about the constituents of a biofilm on a micrometer scale is surface-enhanced Raman spectroscopic imaging. The main goals are:1. Design and synthesis of uniform, highly disperse and sensitive SERS nano-substrates;2. Real time, in vivo, simultaneous and multiplexed imaging studies of EPS constituents using SERS microscopy.

生物膜是自然环境中微生物生活的一种普遍方式。它们几乎可以在任何与水接触的表面生长。除了细胞成分，胞外聚合物（EPS）是生物膜基质的一个主要部分。关于EPS基质的化学分布的详细知识是相关的，例如用于生物杀灭剂的优化、生物降解策略的优化和用于生物废水处理。表面增强拉曼光谱成像是一种在微米尺度上非破坏性地研究生物膜成分的详细化学信息的有力方法。主要目标是：1.均匀、高分散、高灵敏度Sers纳米基底的设计与合成;2.使用Sers显微镜对EPS成分进行真实的实时、体内、同时和多路成像研究。