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Study of receptors, second messengers and DNA binding proteins on noxio usstimulation

受体、第二信使和 DNA 结合蛋白对 noxio us 刺激的研究

基本信息

批准号：
05454551
负责人：
UEDADA Yutaka
金额：
$ 4.1万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454551/
关键词：
GABA GABA_A receptor gamma subunit Substantia gelatinosa Glutamate Primary sensory afferent system AMPA type receptor Pain Pain gamma1 subunit gamma2 subunit trigeminal ganglion coexpression primary afferent system

项目摘要

This study aimed at a demonstration for regulation of trigeminal sensory system by amino acids with using some histochemical technics, in situ hybridization histochemistry (ISH), immunohistochemistry and combination method of immunohistochemistry and retrograde tracer technic. We examined the expression of gamma-aminobutyric acid (GABA)_A receptor gamma1 and gamma2 subunit mRNAs in the rat trigeminal ganglion (TG) by ISH.Most TG cells expressed both gamma1 and gamma2 mRNAs simultaneously. Furthermore, expression of the gamma1 subunit in the primary sensory afferents was confirmed at the protein level by immunohistochemistry using antiserum against the gamma1 subunit. These findings indicate that primary sensory afferents receive GABAergic input, probably axo-axonically via GABA_A receptors expressing gamma1 and gamma2 subunits. We demonstrated that a few neurons projecting to the thalamus in the TNC (trigeminal nucleus caudalis) were labeled by antiserum that recognizes GluR1 and GluR2/3 subunits of the AMPA type receptor. However, expression of the gamma1 and gamma2 subunits of the GABA_A receptors indicated that several neurons projecting to the thalamus in the TNC receive GABAergic inputs via a GABA_A receptor which at contains least gamma1 and/or gamma2 subunits.

本研究旨在利用组织化学、原位杂交组织化学（ISH）、免疫组织化学以及免疫组织化学与逆行示踪技术相结合的方法，论证氨基酸对三叉神经感觉系统的调节作用。用ISH法检测了γ -氨基丁酸（GABA）_A受体γ - 1和γ - 2亚基mrna在大鼠三叉神经节（TG）中的表达。大多数TG细胞同时表达gamma1和gamma2 mrna。此外，利用抗血清免疫组织化学方法在蛋白水平上证实了gamma1亚基在初级感觉传入中的表达。这些发现表明，初级感觉传入可能通过表达gamma1和gamma2亚基的GABA_A受体轴突接收gaba能输入。我们发现，在三叉神经尾核（TNC）中，少数投射到丘脑的神经元被识别AMPA型受体GluR1和GluR2/3亚基的抗血清标记。然而，GABA_A受体的gamma1和gamma2亚基的表达表明，TNC中投射到丘脑的几个神经元通过至少含有gamma1和/或gamma2亚基的GABA_A受体接受gaba能输入。