Synthesis of molecular sensors for in situ use

1)环糊精(CD)衍生物的合成及表面修饰；用硫辛酸修饰的CD在电极表面制备了CD单分子膜。二茂铁可以穿透Cd单层膜，其他客体化合物的加入可以调节膜的通透性。还合成了同时被硫辛酸和二茂铁取代的CD衍生物。2)光敏脂类的合成和表面修饰，制备了含有光敏偶氮苯残基的人造脂类，并评价了它们在LB膜中的光响应。含双烷基链的偶氮苯脂的光异构化反应优于单烷基链取代的偶氮苯脂。合成了冠醚取代偶氮苯脂类化合物，并研究了其离子敏感性和光响应性能。3)基于选择性结合蛋白的表面修饰；亲和素在金属电极表面形成单分子层。在亲和素修饰的电极表面可以制备生物素标记酶的单分子层。

1) Synthesis of cyclodextrin (CD) derivatives and surface modification ; CD monolayrs were prepared on electrode surface using lipoic acid-modified CDs. Ferrocene can penetrate into the CD monolayr membrane, and the permeability can be regulated by the addition of the other guest compounds. CD derivatives which are substituted with lipoic acid and ferrocene simultaneously were also prepared. Self-assembled membranes are now being prepared based on the di-substituted CDs.2) Synthesis of photosensitive lipids and surface modification ; Artificial lipids containing photosensitive azobenzene residues were prepared and their photoresponse in LB membrane was estimated. Photoisomerization reactions of the azobenzene lipids bearing double alkyl chains were superior to those of the single alkyl chain-substituted lipids. Crown ether-substituted azobenzene lipids were also synthesized and ion sensitivity and photoresponse of the LB membranes were studied. The crown ether-substituted lipids exhibited a selective ion binding behavior and photoresponse in the LB membrane.3) Surface modification based on selective binding protein ; Avidin was found to form monomolecular layr on the surface of metal electrodes. Monomolecular layrs of biotin-labeled enzyme can be prepared on the surface of the avidin-modified electrodes.