Synthesis of molecular sensors for in situ use

原位使用分子传感器的合成

• 批准号: 05453134
• 负责人: ANZAI Jun-ichi
• 金额: $ 4.48万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05453134/
• 关键词: Molecular recognition; Surface Modification; Cyclodextrin; Crown ether; LB membrane; Avidin; 分子認識素子; 表面; テオール; 電気化学; 計測

1) Synthesis of cyclodextrin (CD) derivatives and surface modification ; CD monolayrs were prepared on electrode surface using lipoic acid-modified CDs. Ferrocene can penetrate into the CD monolayr membrane, and the permeability can be regulated by the addition of the other guest compounds. CD derivatives which are substituted with lipoic acid and ferrocene simultaneously were also prepared. Self-assembled membranes are now being prepared based on the di-substituted CDs.2) Synthesis of photosensitive lipids and surface modification ; Artificial lipids containing photosensitive azobenzene residues were prepared and their photoresponse in LB membrane was estimated. Photoisomerization reactions of the azobenzene lipids bearing double alkyl chains were superior to those of the single alkyl chain-substituted lipids. Crown ether-substituted azobenzene lipids were also synthesized and ion sensitivity and photoresponse of the LB membranes were studied. The crown ether-substituted lipids exhibited a selective ion binding behavior and photoresponse in the LB membrane.3) Surface modification based on selective binding protein ; Avidin was found to form monomolecular layr on the surface of metal electrodes. Monomolecular layrs of biotin-labeled enzyme can be prepared on the surface of the avidin-modified electrodes.

1)环糊精（CD）衍生物的合成及表面改性；用硫辛酸修饰的CD在电极表面制备了CD单分子膜。二茂铁可以渗透到CD单层膜中，其渗透性可以通过添加其他客体化合物来调节。同时制备了硫辛酸和二茂铁取代的CD衍生物。基于双取代的CDs制备了自组装膜。2)光敏脂质的合成及表面改性；制备了含光敏偶氮苯残基的人工脂质，并对其在LB膜上的光响应进行了评价。含双烷基链偶氮苯脂的光异构化反应优于单烷基链取代的脂类。还合成了冠醚取代偶氮苯脂，并研究了LB膜的离子敏感性和光响应。冠醚取代脂类在LB膜上表现出选择性离子结合行为和光响应。3)基于选择性结合蛋白的表面修饰；发现亲和素在金属电极表面形成单分子层。生物素标记酶的单分子层可以在亲和素修饰的电极表面制备。

项目成果

Jun-ichi Anzai: "Photoinduced reversible trans-cis isomerisation of azobenzene amphiphiles bearing dialkyl side chains in Langmuir-Blodgett membranes." J. Chem. Soc., Perkin Trans. 2. 1897-1898 (1994)

Jun-ichi Anzai：“朗缪尔-布洛杰特膜中带有二烷基侧链的偶氮苯两亲物的光诱导可逆反式顺式异构化。”

Bei Guo: "Adsorption behavior of serum albumin on electrode surfaces and the effects of electrode potential." Chem. Pharm. Bull.44. 800-803 (1996)

郭北：“血清白蛋白在电极表面的吸附行为及电极电位的影响”。

Jun-ichi Anzai: "Photoinduced reversible trans-cis isomerisation of azobenzene amphiphiles bearing dialkyl side chains in Langmuir-Blodgett membranes." J.Chem. Soc., Perkin Trans.2. 1897-1989 (1994)

Jun-ichi Anzai：“朗缪尔-布洛杰特膜中带有二烷基侧链的偶氮苯两亲物的光诱导可逆反式顺式异构化。”

Jun-ichi Anzai: "Avidin-biotin complexation for enzyme sensor applications." Trends Anal. Chem.13. 205-210 (1994)

Jun-ichi Anzai：“用于酶传感器应用的亲和素-生物素络合。”

Jun-ichi Anzai: "Elimination of ascorbate interference of glucose biosensors by use of enzyme multilayers composed of avidin and biotin-labeled glucose oxidase." Denki Kagaku. 63. 1141-1142 (1995)

Jun-ichi Anzai：“通过使用由亲和素和生物素标记的葡萄糖氧化酶组成的酶多层消除葡萄糖生物传感器的抗坏血酸干扰。”