Comparative endocrinological study on hormone receptors related to reproductive function

生殖功能相关激素受体的比较内分泌学研究

• 批准号: 05454021
• 负责人: KAWASHIMA Seiichiro
• 金额: $ 4.1万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454021/
• 关键词: Hormone receptor; cDNA cloning; RT-PCR reaction; Hypothalamus; Pituitary; Gonad; Comparative endocrinology; In situ hybridization; 転写制御

This project aims to clarify the regulatory mechanism of peptide hormone receptors relating reproductive function. The results obtained are as follows.(1)The whole base sequence of cDNA encoding gonadotropin-releasing hormone (GnRH) was analyzed. The expression of mRNA for GnRH receptors in the pituitary is found to be limited to LH and FSH cells. Furthermore, the expression in the ovarian follicles both maturing and atreticindicates the role of GnRH in follicular maturation and atresia.(2)Gonadotropin receptor cDNAs were analyzed in various animal species. Sequencing of extracellular domain was completed in the quail, first discovery in avian species. The determination of epitopes of raised antibody for the extracellular domain was partly completed.Regulation of receptor number by artificial manipulation of photoperiod in wild birds showed the results as expected.(3)Radioreceptor assay revealed that the uterus contains specific prolactin receptors preferentially in the myometrial cells. The prolactin binding capacity of the uterus was elevated by prolactin and tended to increase by estrogen.(4)Immunohistochemical study in dwarf gourami revealed that some GnRH neurons showed isotocin immunoreactivity, indicating functional correlation between GnRH and isotocin.

本项目旨在阐明与生殖功能相关的肽类激素受体的调控机制。所得结果如下：(1)对编码促性腺激素释放激素(GnRH)的cDNA全碱基序列进行了分析。发现垂体中 GnRH 受体 mRNA 的表达仅限于 LH 和 FSH 细胞。此外，GnRH在成熟卵泡和闭锁卵泡中的表达表明GnRH在卵泡成熟和闭锁中的作用。(2)分析了不同动物种属的促性腺激素受体cDNA。细胞外结构域的测序在鹌鹑中完成，这是在鸟类物种中的首次发现。所产生的抗体胞外结构域表位的测定已部分完成。在野鸟中通过人工操纵光周期来调节受体数量，结果符合预期。(3)放射受体测定显示子宫肌层细胞中优先含有特异性催乳素受体。催乳素使子宫的催乳素结合能力升高，而雌激素则有增强的趋势。(4)侏儒吻口鱼的免疫组织化学研究显示，一些GnRH神经元表现出同产素免疫反应性，表明GnRH与同产素之间存在功能相关性。

项目成果

Matsuda, M., T.Mori, M.K.Park and S.Kawashima: "Enhanced cell proliferation by hyperprolactinemia in both exocrine and endocrine pancreas in mice." European Journal of Endocrinology. 130. 187-194 (1994)

Matsuda, M.、T.Mori、M.K.Park 和 S.Kawashima：“高催乳素血症增强小鼠外分泌和内分泌胰腺的细胞增殖。”

Kawashima,S.et al.: "Photoperiodic regulation of follicle-stimulating hormone binding to......21GC01:General and Comparative Endocrinology" 92. 250-259 (1993)

Kawashima,S.et al.：“促卵泡激素结合......21GC01的光周期调节：普通和比较内分泌学”92. 250-259 (1993)

Kawashima,S.and K.Takagi: "Role of sex steroids on the survival,neuritic outgrowth of neurons,and....." Hormones and Behavior. 28. 305-312 (1994)

Kawashima,S. 和 K.Takagi：“性类固醇对神经元生存、神经炎生长的作用，以及......”激素和行为。

Ohta,N.,et al.: "Hormonal modulation of prolyl endopeptidase and dipeptidyl peptidase IV activities in the mouse uterus and ovary." Acta Endocrinologica. 127. 262-266 (1993)

Ohta,N.,et al.：“小鼠子宫和卵巢中脯氨酰内肽酶和二肽基肽酶 IV 活性的激素调节。”

Kawashima, S., K.Tsutsui, R.N.Saxna and S.Ishii: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentration in Indian weaver birds." General and Comparative Endocrinology. 92. 250-259 (1993)

Kawashima, S.、K.Tsutsui、R.N.Saxna 和 S.Ishii：“印度织布鸟促卵泡激素与性腺结合的光周期调节以及血浆促性腺激素浓度的调节”。