Aging of Higher Vertebrates and the Renal and Reproductive Endocrine Systems.

应用形态、内分泌学、生理学、药理学和分子生物学等技术，研究了高等脊椎动物肾脏和生殖内分泌系统包括神经内分泌系统随年龄的变化。在这次合作研究的最后两年中，获得了以下新的发现1。建立了培养的大鼠和小鼠脑内分离细胞内脂褐素蓄积的定量方法。维生素E被证明能有效地减少培养细胞中脂褐素的储存。2.类固醇激素，特别是雌激素和神经生长因子是大鼠视前-下丘脑和边缘系统发育的重要因素。3.为分析高龄大鼠催乳素分泌增加的原因，对培养的大鼠垂体细胞给予多巴胺和多巴胺前体。很明显，对这些药物的反应性有明显的年龄相关变化。4.建立了反向溶血空斑试验，用于分析单个细胞内激素的分泌量。该检测系统能有效地显示多巴胺对催乳素分泌的抑制作用。5.老年大鼠分泌催乳素的自发性腺瘤中催乳素mRNA的含量低于幼年大鼠雌激素诱导的腺瘤。6.老年大鼠生长激素分泌减少与生长激素信使核糖核酸含量减少有关。7.建立了Wistar/Tw大鼠中年多饮多尿的多项指标，包括水代谢、肌酐代谢、血浆加压素和催产素水平。多饮、多尿的发生早于男性和女性。实验结果表明，雄激素加速了这种情况的发生。这项为期两年的合作研究回答了许多最初的问题。然而，出现了几个需要进一步分析的新问题。

Age-related changes of renal and reproductive endocrine systems including the neuroendocrine system in higher vertebrates were studied by means of morphological, endocrinological, physioliogical, harmacological and molecular biological techniques. During the last two years of this co-operative research, the following new findings were obtained.1. The quantitative method of lipofuscin accumulation in cultured dissociated cells from the brain of rats and mice was established. Vitamin E was proved to be effective in reducing the storage of lipofuscin in cultured cells. 2. Steroid hormones, in particular, estrogen and nerve growth factors were proved to be important factors for the development of preoptic-hypothalamic and limbic systems in the rat. 3. In order to analyze the cause of elevated prolactin secretion in the rat at advanced ages, cultured pituitary cells were given dopamine and dopamine precursor. It was evident that there was clear age-related changes in the responsiveness to these agents. 4. Reverse hemolytic plaque assay was established to analyse the amount of hormones secreted in a single cell. The assay system was effective in demonstrating the inhibitory effect of dopamine on prolactin secretion. 5. The amount of prolactin mRNA was less in prolactin-secreting spontaneous adenoma in old rats than estrogen-induced pituitary adenoma in young rats. 6. The decreased secretion of growth hormone in old rats was related to-the decrease in in amount of growth hormone mRNA. 7. Multiple parameters in Wistar/Tw rats showing polydipsia and polyuria at middle ages were established, such as, water metabolism, creatinine metabolism, plasma levels of vasopressin and oxytocin. The occurrence of polydipsia and polyuria was earlier in males and females. Experimental results showed that androgen accelerates the occurrence.Many of the initial questions were answered by this two-year cooperative research. However, several new questions for further analyses have arisen.