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Aging of Higher Vertebrates and the Renal and Reproductive Endocrine Systems.

高等脊椎动物的衰老以及肾脏和生殖内分泌系统。

基本信息

批准号：
63304008
负责人：
KAWASHIMA Seiichiro
金额：
$ 7.04万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63304008/
关键词：
Aging Neuroendocrinology Cell culture Prolactin Growth hormone Messenger RNA Oxytocin Renal function エイジン内分泌器官中枢神経系 LH バソプレシン

项目摘要

Age-related changes of renal and reproductive endocrine systems including the neuroendocrine system in higher vertebrates were studied by means of morphological, endocrinological, physioliogical, harmacological and molecular biological techniques. During the last two years of this co-operative research, the following new findings were obtained.1. The quantitative method of lipofuscin accumulation in cultured dissociated cells from the brain of rats and mice was established. Vitamin E was proved to be effective in reducing the storage of lipofuscin in cultured cells. 2. Steroid hormones, in particular, estrogen and nerve growth factors were proved to be important factors for the development of preoptic-hypothalamic and limbic systems in the rat. 3. In order to analyze the cause of elevated prolactin secretion in the rat at advanced ages, cultured pituitary cells were given dopamine and dopamine precursor. It was evident that there was clear age-related changes in the responsiveness to these agents. 4. Reverse hemolytic plaque assay was established to analyse the amount of hormones secreted in a single cell. The assay system was effective in demonstrating the inhibitory effect of dopamine on prolactin secretion. 5. The amount of prolactin mRNA was less in prolactin-secreting spontaneous adenoma in old rats than estrogen-induced pituitary adenoma in young rats. 6. The decreased secretion of growth hormone in old rats was related to-the decrease in in amount of growth hormone mRNA. 7. Multiple parameters in Wistar/Tw rats showing polydipsia and polyuria at middle ages were established, such as, water metabolism, creatinine metabolism, plasma levels of vasopressin and oxytocin. The occurrence of polydipsia and polyuria was earlier in males and females. Experimental results showed that androgen accelerates the occurrence.Many of the initial questions were answered by this two-year cooperative research. However, several new questions for further analyses have arisen.

通过形态学、内分泌学、生理学、药理学和分子生物学技术研究高等脊椎动物肾脏和生殖内分泌系统（包括神经内分泌系统）与年龄相关的变化。经过近两年的合作研究，取得了以下新成果： 1．建立了大鼠和小鼠脑培养分离细胞脂褐质积累的定量方法。维生素E被证明可以有效减少培养细胞中脂褐质的储存。 2、类固醇激素，特别是雌激素和神经生长因子被证明是大鼠视前下丘脑和边缘系统发育的重要因素。 3.为了分析高龄大鼠催乳素分泌升高的原因，对培养的垂体细胞给予多巴胺和多巴胺前体。很明显，对这些药物的反应存在明显的与年龄相关的变化。 4.建立反向溶血斑测定法，分析单个细胞分泌的激素量。该测定系统有效地证明了多巴胺对催乳素分泌的抑制作用。 5.老年大鼠自发性催乳素腺瘤中催乳素mRNA含量较雌激素诱发的幼年大鼠垂体腺瘤少。 6、老年大鼠生长激素分泌减少与生长激素mRNA量减少有关。 7.建立了中年多饮多尿Wistar/Tw大鼠的水代谢、肌酐代谢、血浆加压素和催产素水平等多项参数。男性和女性多饮、多尿发生较早。实验结果表明，雄激素加速了这一发生。这项为期两年的合作研究回答了许多最初的问题。然而，出现了一些需要进一步分析的新问题。