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気中汚染物質に対する植物防御の統合的システムに関する分子生理学的研究

植物防御空气污染物综合系统的分子生理学研究

基本信息

批准号：
18F18391
负责人：
森泉
金额：
$ 1.47万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2018
资助国家：
日本
起止时间：
2018-11-09 至 2021-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-18F18391/
关键词：
Sulfur dioxide plants stomata abiotic stress defence mechanism sulfur oxide

项目摘要

Sulfur dioxide is a toxic gas. Plants has a mechanism to prevent the invasion of sulfur oxide gas into the internal tissue by closing stomata. Stomata is a microscopic opening formed by a pair of guard cells on leaf surface in plants.Some plant growth regulators are also postulated to participate in sulfur dioxide signaling in guard cells by earlier studies, while its rationale was obscure. In this study, we employed quantitative analysis of plant growth regulators, gibberellins, auxin, cytokinines, abscisic acid, jasmonates, and salicylic acid, by liquid chromatography-mass spectrometry. We identified that contents of jasmonates. Therefore, we examined the movement of stomata in a jasmonate-insensitive mutant. Contrary to our expectation, stomata of thejasmonate-insensitive mutant did not show an impairment in sulfur dioxide response, indicating that jasmonate does not participate to sulfur dioxide-induced stomatal closure.Beside above experiment, we have questioned whether cytosolic acidification participates in sulfur dioxide signaling in guard cells or not. We employed an analysis of the mutants, which have stabilized pH of guard cells by mutation in CLCa gene. The mutants of CLCa showed an intensified resistance to sulfur dioxide in terms of stomatal closure induction as well as cell death of guard cells. This supports the idea that cytosolic acidification participates in sulfur dioxide signaling in guard cell leading to stomatal closure. At the same time this provides a potential approach to generate sulfur dioxide-resistant crops.

二氧化硫是一种有毒气体。植物具有通过关闭气孔来防止硫氧化物气体侵入内部组织的机制。气孔是植物叶片表面由一对保卫细胞形成的微小开口，早期的研究也认为一些植物生长调节物质参与了保卫细胞的SO2信号转导，但其机理尚不清楚。在这项研究中，我们采用定量分析植物生长调节剂，赤霉素，生长素，细胞分裂素，脱落酸，茉莉酸，水杨酸，液相色谱-质谱。我们确定了茉莉酸酯的含量。因此，我们研究了茉莉酸不敏感突变体的气孔运动。与我们的预期相反，茉莉酸不敏感突变体的气孔没有表现出对SO2反应的损伤，表明茉莉酸不参与SO2诱导的气孔关闭。我们对通过CLCa基因突变稳定保卫细胞pH的突变体进行了分析。CLCa突变体在诱导气孔关闭和保卫细胞死亡方面表现出对二氧化硫的强烈抗性。这支持了胞质酸化参与保卫细胞中二氧化硫信号传导导致气孔关闭的观点。同时，这为产生抗二氧化硫作物提供了一种潜在的方法。