Deciphering the role of intra and extracellular elasticity for protrusion growth, stability and switching during cell motility in vivo.

解读细胞内和细胞外弹性对体内细胞运动过程中突起生长、稳定性和转换的作用。

• 批准号: 394329438
• 负责人: Dr. Alba Diz-Muñoz
• 金额: --
• 依托单位: Europäisches Laboratorium für Molekularbiologie (EMBL)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/394329438?language=en
• 关键词: Deciphering; role; intra; extracellular; elasticity

Cell protrusions such as lamellipodia play a central role in cell migration. During development and cancer cell migration, switching between different protrusion types has been observed. Cell mechanics, especially membrane-to-cortex attachment, contractility, cell adhesion, and actin polymerization have been shown to be important for protrusion formation. The contributions of other physical parameters such as intra and extracellular elasticity are still poorly understood due to the lack of convenient assay systems and the absence of technology to measure mechanical properties in a contactless way in vivo. In this proposal, we aim to understand the role of intra and extracellular elasticity in protrusion growth, stability, and switching in vivo. We will initially test the role of leading edge elasticity in lamellipodia formation ex vivo. Next, we will establish and benchmark Brillouin microscopy as a tool that can measure elastic properties in 3D environments. Lastly, we will use Brillouin Light Scattering Spectroscopy (BLSS) combined with fluorescence microscopy to decipher the role of intra and extracellular elasticity in protrusion growth, stability and switching during zebrafish mesendodermal migration in vivo.

细胞突起如板足在细胞迁移中起核心作用。在癌细胞的发育和迁移过程中，可以观察到不同类型的突起之间的切换。细胞力学，特别是膜与皮层的附着、收缩性、细胞粘附和肌动蛋白聚合已被证明对突起的形成很重要。由于缺乏方便的分析系统和缺乏以非接触方式测量体内机械性能的技术，其他物理参数（如细胞内和细胞外弹性）的贡献仍然知之甚少。在本研究中，我们旨在了解细胞内和细胞外弹性在体内突起生长、稳定和转换中的作用。我们将首先测试前缘弹性在板足形成中的作用。接下来，我们将建立和基准布里渊显微镜作为一种工具，可以在3D环境中测量弹性特性。最后，我们将使用布里louin光散射光谱（BLSS）结合荧光显微镜来破译斑马鱼体内中内皮迁移过程中细胞内和细胞外弹性在突起生长、稳定性和切换中的作用。

项目成果

A 3D Brillouin microscopy dataset of the in-vivo zebrafish eye

体内斑马鱼眼睛的 3D 布里渊显微镜数据集

• DOI: 10.1016/j.dib.2020.105427
• 发表时间: 2020
• 期刊: Data in Brief
• 影响因子: 1.2
• 作者: Sánchez-Iranzo H;Bevilacqua C;Diz-Muñoz A;Prevedel R
• 通讯作者: Prevedel R

Protocol on Tissue Preparation and Measurement of Tumor Stiffness in Primary and Metastatic Colorectal Cancer Samples with an Atomic Force Microscope

• DOI: 10.1016/j.xpro.2020.100167
• 发表时间: 2020-11-16
• 期刊: STAR Protocols
• 作者: Shen Y;Schmidt T;Diz-Muñoz A
• 通讯作者: Diz-Muñoz A